Document Detail

Are routine ancillary stains required to diagnose Helicobacter infection in gastric biopsy specimens? An institutional quality assurance review.
MedLine Citation:
PMID:  22261451     Owner:  NLM     Status:  MEDLINE    
Gastric biopsies are often done to evaluate for Helicobacter gastritis. Given the oncogenic association with Helicobacter gastritis and the relative ease of therapy, it is important for pathology departments to identify all positive cases. We describe an institutional quality assurance study of an institutional method for the diagnosis of Helicobacter gastritis. We reviewed 356 gastric biopsy specimens from a 4-week period at 1 institution. Approximately half were evaluated by 4 methods, H&E stain, Giemsa stain, Warthin-Starry stain, and Helicobacter immunostain, while the remainder were stained only with H&E and Helicobacter immunostains. There were 30 cases of Helicobacter gastritis diagnosed; about 83% of cases were diagnosed on the initial H&E-stained slides. Our study highlights a quality assurance study and a head-to head comparison of 4 methods not previously reported and supports the use of ancillary stains at the discretion of the sign-out pathologist.
Douglas J Hartman; Scott R Owens
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  137     ISSN:  1943-7722     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-20     Completed Date:  2012-03-06     Revised Date:  2012-03-22    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  255-60     Citation Subset:  AIM; IM    
Dept of Pathology, University of Pittsburgh Medical Center, 200 Lothrop Street, Pittsburgh, PA 15213, USA. .
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MeSH Terms
Aged, 80 and over
Gastric Mucosa / microbiology,  pathology*
Gastritis / diagnosis*,  microbiology
Helicobacter Infections / diagnosis*,  microbiology
Helicobacter pylori / isolation & purification
Middle Aged
Quality Assurance, Health Care*
Staining and Labeling / methods*,  standards
Young Adult
Comment In:
Am J Clin Pathol. 2012 Mar;137(3):343-5   [PMID:  22338045 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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