Document Detail

Are prostanoids related to positive inotropism by UTP and ATP?
MedLine Citation:
PMID:  15564788     Owner:  NLM     Status:  MEDLINE    
Uridine 5'-triphosphate (UTP) and adenosine 5'-triphosphate (ATP) induce biphasic inotropic effects: first a decrease and then an increase in contractile tension were observed in isolated rat myocardial tissues. Inotropic effects were higher in atrial tissue than in ventricular or papillary muscle; thus, experiments were mostly carried out on rat atria. In this research, we mainly studied positive inotropism by using selective inhibitors of the arachidonic acid cascade. The natural compounds luffariellolide and aristolochic acid, two inhibitors of PLA2, both inhibited positive inotropism by UTP but not by ATP, whereas they did not modify their negative inotropism. Indomethacin (5 micromol/l), an inhibitor of COX-1, reduced positive inotropism by UTP but not by ATP, without modifying their negative inotropism. Nimesulide (1 micromol/l), an inhibitor of COX-2, did not change any of the effects caused by nucleotides. Nor did NDGA (10 micromol/l), an inhibitor of lipoxygenase, change inotropism by nucleotides. Arachidonic acid pretreatment (10 micromol/l) increased inotropic effects by UTP without affecting those of ATP. These data suggest that there are differences in the mechanisms responsible for the positive inotropism caused by UTP in comparison with ATP; the effect of UTP depends on PLA2 activation and PG(s) release, whereas that of ATP does not.
Guglielmina Froldi; Giampietro Galzignato; Miriam Zanetti; Monica Montopoli; Paola Dorigo; Laura Caparrotta
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article     Date:  2004-11-24
Journal Detail:
Title:  Pharmacology     Volume:  73     ISSN:  0031-7012     ISO Abbreviation:  Pharmacology     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-04     Completed Date:  2005-04-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0152016     Medline TA:  Pharmacology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  140-5     Citation Subset:  IM    
Copyright Information:
Copyright 2005 S. Karger AG, Basel.
Department of Pharmacology and Anaesthesiology, University of Padova, Padova, Italy.
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MeSH Terms
Adenosine Triphosphate / pharmacology*
Myocardial Contraction / drug effects*,  physiology
Prostaglandins / pharmacology
Rats, Wistar
Stimulation, Chemical
Uridine Triphosphate / pharmacology*
Reg. No./Substance:
0/Prostaglandins; 56-65-5/Adenosine Triphosphate; 63-39-8/Uridine Triphosphate

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