Document Detail


Are globoseries glycosphingolipids SSEA-3 and -4 markers for stem cells derived from human umbilical cord blood?
MedLine Citation:
PMID:  21149348     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Umbilical cord blood (UCB) is an efficient and valuable source of hematopoietic stem cells (HSCs) for transplantation. In addition to HSCs it harbours low amounts of mesenchymal stem cells (MSCs). No single marker to identify cord blood-derived stem cells, or to indicate their multipotent phenotype, has been characterized so far. SSEA-3 and -4 are cell surface globoseries glycosphingolipid epitopes that are commonly used as markers for human embryonic stem cells, where SSEA-3 rapidly disappears when the cells start to differentiate. Lately SSEA-3 and -4 have also been observed in MSCs. As there is an ongoing discussion and variation of stem-cell markers between laboratories, we have now comprehensively characterized the expression of these epitopes in both the multipotent stem-cell types derived from UCB. We have performed complementary analysis using gene expression analysis, mass spectrometry and immunochemical methods, including both flow cytometry and immunofluoresence microscopy. SSEA-4, but not SSEA-3, was expressed on MSCs but absent from HSCs. Our findings indicate that SSEA-3 and/or -4 may not be optimal markers for multipotency in the case of stem cells derived from cord blood, as their expression may be altered by cell-culture conditions.
Authors:
Heli Suila; Virve Pitkänen; Tia Hirvonen; Annamari Heiskanen; Heidi Anderson; Anita Laitinen; Suvi Natunen; Halina Miller-Podraza; Tero Satomaa; Jari Natunen; Saara Laitinen; Leena Valmu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-12
Journal Detail:
Title:  Journal of molecular cell biology     Volume:  3     ISSN:  1759-4685     ISO Abbreviation:  J Mol Cell Biol     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-04     Completed Date:  2011-07-12     Revised Date:  2011-12-29    
Medline Journal Info:
Nlm Unique ID:  101503669     Medline TA:  J Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  99-107     Citation Subset:  IM    
Affiliation:
Research and Development, Finnish Red Cross Blood Service, R&D, Kivihaantie 7, FIN-00310 Helsinki, Finland.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Tumor-Associated, Carbohydrate / genetics,  metabolism*
Cell Differentiation
Cells, Cultured
Fetal Blood / cytology,  metabolism*
Flow Cytometry
Gene Expression
Glycosphingolipids / metabolism*
Hematopoietic Stem Cells / cytology,  metabolism*
Humans
Mesenchymal Stem Cells / cytology,  metabolism*
Stage-Specific Embryonic Antigens / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Antigens, Tumor-Associated, Carbohydrate; 0/Glycosphingolipids; 0/Stage-Specific Embryonic Antigens; 0/stage-specific embryonic antigen-3; 0/stage-specific embryonic antigen-4

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