Document Detail

Are exercise-induced genes induced by exercise?
MedLine Citation:
PMID:  15516373     Owner:  NLM     Status:  MEDLINE    
Numerous human in vivo studies on skeletal muscle gene expression have investigated the effects of given interventions. These have been founded on the assumption that presampling can be regarded as a representative control for postintervention sampling. However, many genes are responsive to the metabolic status, which varies during the day, so that observed differences in gene expression between the pre- and post-sample may therefore be a result of the daily variations rather than an intervention. Furthermore, the sampling itself can cause a local stress response, which may also influence the expression of some genes in later samples from the same localized area. To test this, we performed a short-term human endurance exercise study in which muscle biopsies were obtained from healthy untrained individuals (n=14) before and in the hours after exercise to measure the expression of mRNA for previously reported exercise-related genes (e.g., PPARgamma coactivator-1alpha (PGC-1alpha), pyruvate dehydrogenase kinase 4 (PDK4), MyoD, p21, (heat shock protein 72 (HSP72), lipoprotein lipase (LPL), citrate synthase (CS), and glucose transporter 4 (GLUT4)). To test for changes unrelated to exercise, one half of the subjects did not exercise. As suspected, several presumed exercise-induced genes were induced even without the exercise. Our data demonstrate that presampling is not always a representative control for postintervention sampling, illustrating that use of presampling can cause erroneous interpretations of the underlying induction signals.
Kristian Vissing; Jesper L Andersen; Peter Schjerling
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-10-29
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  19     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-04     Completed Date:  2005-11-07     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  94-6     Citation Subset:  IM    
Department of Molecular Muscle Biology, Copenhagen Muscle Research Centre, Rigshospitalet, Copenhagen, Denmark.
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MeSH Terms
Citrate (si)-Synthase / genetics
Exercise / physiology*
Gene Expression Regulation / physiology*
Genes, cdc / physiology
Glucose Transporter Type 4
HSP72 Heat-Shock Proteins
Heat-Shock Proteins / genetics
Lipoprotein Lipase / genetics
Monosaccharide Transport Proteins / genetics
Muscle Proteins / genetics
Muscle, Skeletal / chemistry,  metabolism
MyoD Protein / genetics
Myogenin / genetics
Oxidative Stress
Protein Kinases / genetics
Proto-Oncogene Proteins p21(ras) / genetics
Transcription Factors / genetics
Reg. No./Substance:
0/Glucose Transporter Type 4; 0/HSP72 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/MYOG protein, human; 0/Monosaccharide Transport Proteins; 0/Muscle Proteins; 0/MyoD Protein; 0/Myogenin; 0/PPARGC1A protein, human; 0/SLC2A4 protein, human; 0/Transcription Factors; EC (si)-Synthase; EC 2.7.-/Protein Kinases; EC 2.7.1.-/pyruvate dehydrogenase kinase 4; EC Lipase; EC protein, human; EC Proteins p21(ras)

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