Document Detail


Are exercise-induced genes induced by exercise?
MedLine Citation:
PMID:  15516373     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Numerous human in vivo studies on skeletal muscle gene expression have investigated the effects of given interventions. These have been founded on the assumption that presampling can be regarded as a representative control for postintervention sampling. However, many genes are responsive to the metabolic status, which varies during the day, so that observed differences in gene expression between the pre- and post-sample may therefore be a result of the daily variations rather than an intervention. Furthermore, the sampling itself can cause a local stress response, which may also influence the expression of some genes in later samples from the same localized area. To test this, we performed a short-term human endurance exercise study in which muscle biopsies were obtained from healthy untrained individuals (n=14) before and in the hours after exercise to measure the expression of mRNA for previously reported exercise-related genes (e.g., PPARgamma coactivator-1alpha (PGC-1alpha), pyruvate dehydrogenase kinase 4 (PDK4), MyoD, p21, (heat shock protein 72 (HSP72), lipoprotein lipase (LPL), citrate synthase (CS), and glucose transporter 4 (GLUT4)). To test for changes unrelated to exercise, one half of the subjects did not exercise. As suspected, several presumed exercise-induced genes were induced even without the exercise. Our data demonstrate that presampling is not always a representative control for postintervention sampling, illustrating that use of presampling can cause erroneous interpretations of the underlying induction signals.
Authors:
Kristian Vissing; Jesper L Andersen; Peter Schjerling
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-10-29
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  19     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-04     Completed Date:  2005-11-07     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  94-6     Citation Subset:  IM    
Affiliation:
Department of Molecular Muscle Biology, Copenhagen Muscle Research Centre, Rigshospitalet, Copenhagen, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Adult
Citrate (si)-Synthase / genetics
Exercise / physiology*
Gene Expression Regulation / physiology*
Genes, cdc / physiology
Glucose Transporter Type 4
HSP72 Heat-Shock Proteins
Heat-Shock Proteins / genetics
Humans
Lipoprotein Lipase / genetics
Male
Monosaccharide Transport Proteins / genetics
Muscle Proteins / genetics
Muscle, Skeletal / chemistry,  metabolism
MyoD Protein / genetics
Myogenin / genetics
Oxidative Stress
Protein Kinases / genetics
Proto-Oncogene Proteins p21(ras) / genetics
Transcription Factors / genetics
Chemical
Reg. No./Substance:
0/Glucose Transporter Type 4; 0/HSP72 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/MYOG protein, human; 0/Monosaccharide Transport Proteins; 0/Muscle Proteins; 0/MyoD Protein; 0/Myogenin; 0/PPARGC1A protein, human; 0/SLC2A4 protein, human; 0/Transcription Factors; EC 2.3.3.1/Citrate (si)-Synthase; EC 2.7.-/Protein Kinases; EC 2.7.1.-/pyruvate dehydrogenase kinase 4; EC 3.1.1.34/Lipoprotein Lipase; EC 3.6.5.2/HRAS protein, human; EC 3.6.5.2/Proto-Oncogene Proteins p21(ras)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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