Document Detail

Are all lyssavirus genes equal for phylogenetic analyses?
MedLine Citation:
PMID:  17681631     Owner:  NLM     Status:  MEDLINE    
Individual lyssavirus genes were evaluated for phylogenetic studies from available full genome sequences. The full genome of the ERA rabies virus was sequenced and its accuracy was confirmed through virus recovery by reverse genetics. The full length of the ERA is 11,931 nucleotides (nt), with a leader sequence of 58 nt, the nucleoprotein (N) gene of 1350 nt, phosphoprotein (P) gene of 891 nt, matrix protein (M) gene of 606 nt, glycoprotein (G) gene of 1572 nt, RNA-dependent RNA polymerase (L) gene of 6384 nt, Psi-region (or G-L intergenic region) of 400 nt, and a trailer region of 70 nt. The five mono-cistrons are separated by intergenic regions of 2, 5, 5 and 24 nt, respectively. One obvious difference between the ERA and SAD-B19 rabies virus strains was the putative stop/polyadenylation signal of the G gene, with a poly(A(8)) tract for ERA, and a poly(A(5)) for SAD-B19. The TGpoly(A(8)) sequence tract was identified to be a leaky termination signal in the ERA strain. Through analyses of nt diversity, protein co-variations, structural and functional constraints, and reconstruction of phylogenetic trees from comprehensive datasets, we propose lyssavirus genes probably are of similar value for phylogenetic analyses.
Xianfu Wu; Richard Franka; Andres Velasco-Villa; Charles E Rupprecht
Publication Detail:
Type:  Journal Article     Date:  2007-08-02
Journal Detail:
Title:  Virus research     Volume:  129     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-23     Completed Date:  2008-01-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  91-103     Citation Subset:  IM    
Rabies Program/PRB, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cell Line
Genes, Viral*
Genome, Viral
Molecular Sequence Data
Rabies virus / classification*,  genetics*
Sequence Alignment
Viral Proteins / chemistry,  genetics
Reg. No./Substance:
0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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