| Are cutaneous microdialysis cytokine findings supported by end point biopsy immunohistochemistry findings? | |
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MedLine Citation:
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PMID: 20967522 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Insertion of a cutaneous microdialysis catheter into normal dermis has been shown to induce the production of IL1b, IL6 and IL8 in an innate response to minimal trauma. In the present study, skin biopsy for immunohistochemistry has been performed at the site of the microdialysis catheter to compare the findings with that of the microdialysis findings 24 h after insertion. Of the three named cytokines, concordance between the two investigated technologies was highest for IL8 (100%) followed by IL6 (70%) and IL1b (50%). For seven other pro-inflammatory and T cell-relevant cytokines studied, concordance ranged between 50% and 80%. The total number of positive (microdialysis or immunofluorescence) findings was similar between the two methodologies. Technical and biological phenomenon can explain the differences. We conclude that both methodologies illustrate important features of tissue biology and that a combination of the two methods in clinical research can provide the chronology of soluble mediator participation and the more classic, but also more invasive, biopsy-based methodology at a point which constitutes the end of the observation period. We conclude further that at the 24-h time period here studied, microdialysis catheters are still functional and thus capable of producing relevant data which can be corroborated and extended by the "end point biopsy". |
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Authors:
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Florence Sjögren; Chris D Anderson |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-22 |
Journal Detail:
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Title: The AAPS journal Volume: 12 ISSN: 1550-7416 ISO Abbreviation: AAPS J Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-10 Completed Date: 2011-04-05 Revised Date: 2011-10-24 |
Medline Journal Info:
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Nlm Unique ID: 101223209 Medline TA: AAPS J Country: United States |
Other Details:
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Languages: eng Pagination: 741-9 Citation Subset: IM |
Affiliation:
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Department of Clinical and Experimental Medicine, Linköping University, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biopsy Cytokines / metabolism* Humans Immunohistochemistry Microdialysis Skin / metabolism*, pathology |
| Chemical | |
Reg. No./Substance:
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0/Cytokines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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