| Archaebacterial lipid membranes as models to study the interaction of 10-N-nonyl acridine orange with phospholipids. | |
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MedLine Citation:
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PMID: 18938147 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The dye 10-N-nonyl acridine orange (NAO) is used to label cardiolipin domains in mitochondria and bacteria. The present work represents the first study on the binding of NAO with archaebacterial lipid membranes. By combining absorption and fluorescence spectroscopy with fluorescence microscopy studies, we investigated the interaction of the dye with (a) authentic standards of archaebacterial cardiolipins, phospholipids and sulfoglycolipids; (b) isolated membranes; (c) living cells of a square-shaped extremely halophilic archaeon. Absorption and fluorescence spectroscopy data indicate that the interaction of NAO with archaebacterial cardiolipin analogues is similar to that occurring with diacidic phospholipids and sulfoglycolipids, suggesting as molecular determinants for NAO binding to archaebacterial lipids the presence of two acidic residues or a combination of acidic and carbohydrate residues. In agreement with absorption spectroscopy data, fluorescence data indicate that NAO fluorescence in archaeal membranes cannot be exclusively attributed to bisphosphatidylglycerol and, therefore, different from mitochondria and bacteria, the dye cannot be used as a cardiolipin specific probe in archaeal microorganisms. |
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Authors:
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Simona Lobasso; Matilde Sublimi Saponetti; Francesco Polidoro; Patrizia Lopalco; Jasna Urbanija; Veronika Kralj-Iglic; Angela Corcelli |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-30 |
Journal Detail:
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Title: Chemistry and physics of lipids Volume: 157 ISSN: 1873-2941 ISO Abbreviation: Chem. Phys. Lipids Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-16 Completed Date: 2009-03-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0067206 Medline TA: Chem Phys Lipids Country: Ireland |
Other Details:
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Languages: eng Pagination: 12-20 Citation Subset: IM |
Affiliation:
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Department of Medical Biochemistry, Medical Biology and Medical Physics, University of Bari, P.zza G. Cesare, I-70124 Bari, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acridine Orange
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analogs & derivatives*,
metabolism Animals Archaea / cytology*, metabolism Cattle Cell Membrane / metabolism* Coloring Agents / metabolism* Fluorescence Halobacteriaceae / cytology, metabolism Liposomes / metabolism Mitochondria / metabolism Models, Biological* Phospholipids / metabolism* Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Coloring Agents; 0/Liposomes; 0/Phospholipids; 65-61-2/Acridine Orange; 78125-98-1/N(10)-nonylacridine orange |
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