Document Detail

Arachidonic acid stimulates steroidogenesis in goldfish preovulatory ovarian follicles.
MedLine Citation:
PMID:  2106468     Owner:  NLM     Status:  MEDLINE    
The possibility that arachidonic acid (AA) plays a role in the regulation of steroidogenesis in goldfish was investigated using preovulatory ovarian follicles incubated in vitro. AA was shown to act in a time- and dose-dependent manner to stimulate testosterone production. AA in the range of 10(-5) to 10(-4) M increased testosterone production within 2 hr and had a maximal effect by 9 hr. The magnitude of the testosterone response to AA was similar to that observed when ovarian follicles were incubated with human chorionic gonadotropin (hCG). Ovarian follicles incubated with AA and either hCG or forskolin (adenylate cyclase activator) produced more testosterone than follicles incubated with either of these compounds alone. The actions of AA on testosterone production were completely blocked by cyclooxygenase inhibitors (indomethacin or ibuprofen) and were reduced by 50% by the lipoxygenase inhibitor nordihydroguaiaretic acid. Phospholipase C was far more effective than phospholipase A2 in the stimulation of testosterone production. Taken together, these results suggest that AA formed subsequent to the action of phospholipase C on membrane phospholipids has a role in the regulation of steroidogenesis in preovulatory goldfish ovarian follicles.
G Van der Kraak; J P Chang
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  General and comparative endocrinology     Volume:  77     ISSN:  0016-6480     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  1990 Feb 
Date Detail:
Created Date:  1990-04-12     Completed Date:  1990-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  221-8     Citation Subset:  IM    
Department of Zoology, University of Guelph, Ontario, Canada.
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MeSH Terms
Arachidonic Acid
Arachidonic Acids / pharmacology*
Chorionic Gonadotropin / pharmacology
Cyprinidae / metabolism*
Forskolin / pharmacology
Goldfish / metabolism*
Ovarian Follicle / drug effects,  metabolism*
Phospholipases / pharmacology
Testosterone / biosynthesis*
Reg. No./Substance:
0/Arachidonic Acids; 0/Chorionic Gonadotropin; 506-32-1/Arachidonic Acid; 58-22-0/Testosterone; 66428-89-5/Forskolin; EC 3.1.-/Phospholipases

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