| Arachidonic acid production by the red alga Porphyridium cruentum. | |
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MedLine Citation:
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PMID: 18548719 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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The single-celled alga, Porphyridium cruentum, was assessed by means of chromatographic separation and mass spectral analysis of its fatty acids to be a potentially competetive source of arachidonic (5,8,11,14-eicosatetraenoic) acid. Models for both cell growth and production of the prostaglandin precursor at various temperatures and light intensities are presented. Increasing the light intensity within the range 1700-8000 lux increases the cell growth rate without affecting the arachidonic acid yield per cell; increasing the cultivation temperature from 18 degrees C to ca. 32 degrees C lowers the yield of arachidonic acid per cell but increases the rate of its production per unit volume and time. The increase of the weight ratio of arachidonic:palmitic acids at low temperatures is interpreted as a means of controlling the microviscosities of cellular membranes. In addition, the arachidonic acid content of cells decreases with the culture's age, despite increases in unit cell dry weight. The maximum rate of 0.46 mg arachidonic acid L(-1) h(-1) was calculated by means of the model to occur at ca. 32 degrees C and 8000 lux in liquid cultures of 12 x 10(9) cells/L. Estimates of the cost of producing arachidonic acid by means of this alga range from $0.15/g to $1.00/g of arachidonic acid. Cells grown at 18 degrees C in the presence of 0.3% linoleic acid swelled and produced gorlic (13-cyclopent-2-enyltridec-6-enoic) acid and another compound not normally observed. An estimated threefold increase of arachidonic acid content also occurred, but no significant lipogenesis was induced at 23 degrees C in the presence of 1% kerosene or 0.3% palmitic, stearic, oleic, or linoleic acids. |
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Authors:
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T J Ahern; S Katoh; E Sada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 25 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 1983 Apr |
Date Detail:
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Created Date: 2008-06-12 Completed Date: 2009-12-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 1057-70 Citation Subset: - |
Affiliation:
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Department of Chemical Engineering, Kyoto University, Kyoto 606 Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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