| Arachidonic acid pools of rat kidney cell nuclei. | |
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MedLine Citation:
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PMID: 20838858 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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We have assessed that nuclear lipids from rat kidney cells are not only membrane components, but they are also found within the nucleus. The most abundant nuclear and endonuclear lipids have a high proportion of unsaturated fatty acids (n-6 series: arachidonic > linoleic), mainly esterified to PtdCho. Nuclear most abundant molecular species are 16:0-20:4, 16:0-18:2, 18:0-20:4, 18:0-18:2, and 16:0-18:1. Arachidonic acid is esterified at the sn-2 position of PtdCho: 16:0-20:4(25%), 18:0-20:4(15%), 18:2-20:4(3%), 18:1-20:4(2%). Exogenous [1-(14)C]20:4n-6-CoA is esterified in vitro in GP (glycerophospholipids) > > TAG and DAG. Five PtdCho molecular species were labeled: 16:0-20:4, 18:0-20:4, 18:1-20:4, 18:2-20:4, and 20:4-20:4. In conclusion, these results demonstrated that: (1) there is an important lipid pool within kidney cell nuclei; (2) main nuclear and endonuclear lipid pools were PtdCho molecular species which contained a high proportion of unsaturated fatty acids (20:4n-6 and 18:2n-6) esterified at sn-2 position and 16:0 esterified at sn-1 position; (3) kidney cell nuclei also contained the necessary enzymes to esterify exogenous 20:4n-6-CoA to glycerolipids and to GP; (4) exogenous 20:4n-6-CoA was esterified in five PtdCho molecular species with 20:4n-6 at the sn-2 position, although the most actively synthesized PtdCho contained 20:4n-6 at both the sn-1 and sn-2 positions of the molecule; (5) we can infer that by a remodeling process, the unsaturated fatty acids at the sn-1 position of PtdCho molecular species could be replaced by 16:0 and 18:0, and thus PtdCho would achieve the physiological profile characteristic of the organ. |
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Authors:
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Sabina M Maté; Juan P Layerenza; Ana Ves-Losada |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-14 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 345 ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 259-70 Citation Subset: IM |
Affiliation:
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Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP, CCT-La Plata, CONICET-UNLP), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Calles 60 y 120, 1900, La Plata, Argentina. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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