| Arachidonic acid metabolism regulates Escherichia coli penetration of the blood-brain barrier. | |
| | |
MedLine Citation:
|
PMID: 20696828 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Escherichia coli K1 meningitis occurs following penetration of the blood-brain barrier, but the underlying mechanisms involved in E. coli penetration of the blood-brain barrier remain incompletely understood. We have previously shown that host cytosolic phospholipase A(2)α (cPLA(2)α) contributes to E. coli invasion of human brain microvascular endothelial cells (HBMEC), which constitute the blood-brain barrier, but the underlying mechanisms remain unclear. cPLA(2)α selectively liberates arachidonic acid from membrane phospholipids. Here, we provide the first direct evidence that host 5-lipoxygenase and lipoxygenase products of arachidonic acid, cysteinyl leukotrienes (LTs), contribute to E. coli K1 invasion of HBMEC and penetration into the brain, and their contributions involve protein kinase C alpha (PKCα). These findings demonstrate that arachidonic acid metabolism regulates E. coli penetration of the blood-brain barrier, and studies are needed to further elucidate the mechanisms involved with metabolic products of arachidonic acid for their contribution to E. coli invasion of the blood-brain barrier. |
| | |
Authors:
|
Longkun Zhu; Ravi Maruvada; Adam Sapirstein; Kafait U Malik; Marc Peters-Golden; Kwang Sik Kim |
Related Documents
:
|
8702588 - Role of oxoproline in the regulation of neutral amino acid transport across the blood-b... 6123518 - Simultaneous determination of monoamine transmitters, precursors and metabolites in a s... 6782498 - Role of carbon dioxide fixation, blood aspartate and glutamate in the adaptation of amp... 6653618 - Pharmacokinetics of arginine and aspartic acid administered simultaneously in the rat--... 17940158 - Supplemental algal meal alters the ruminal trans-18:1 fatty acid and conjugated linolei... 8464348 - Hypolipidemic effects of beta-cyclodextrin in the hamster and in the genetically hyperc... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-08-09 |
Journal Detail:
|
Title: Infection and immunity Volume: 78 ISSN: 1098-5522 ISO Abbreviation: Infect. Immun. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-09-22 Completed Date: 2010-11-12 Revised Date: 2011-07-27 |
Medline Journal Info:
|
Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
|
Languages: eng Pagination: 4302-10 Citation Subset: IM |
Affiliation:
|
Division of Pediatric Infectious Diseases, Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arachidonate 5-Lipoxygenase / metabolism Arachidonic Acid / metabolism* Blood-Brain Barrier / microbiology, physiology* Brain / blood supply Endothelial Cells / metabolism* Escherichia coli / physiology* Group IV Phospholipases A2 / metabolism Humans Leukotrienes / metabolism Mice Mice, Knockout Protein Kinase C-alpha / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
AI84984/AI/NIAID NIH HHS; NS26310/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Leukotrienes; 506-32-1/Arachidonic Acid; EC 1.13.11.34/Arachidonate 5-Lipoxygenase; EC 2.7.11.13/Protein Kinase C-alpha; EC 3.1.1.4/Group IV Phospholipases A2 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Survival defects of Cryptococcus neoformans mutants exposed to human cerebrospinal fluid result in a...
Next Document: The staphylococcus-specific gene rsr represses agr and virulence in Staphylococcus aureus.