Document Detail

Arachidonic acid metabolism regulates Escherichia coli penetration of the blood-brain barrier.
MedLine Citation:
PMID:  20696828     Owner:  NLM     Status:  MEDLINE    
Escherichia coli K1 meningitis occurs following penetration of the blood-brain barrier, but the underlying mechanisms involved in E. coli penetration of the blood-brain barrier remain incompletely understood. We have previously shown that host cytosolic phospholipase A(2)α (cPLA(2)α) contributes to E. coli invasion of human brain microvascular endothelial cells (HBMEC), which constitute the blood-brain barrier, but the underlying mechanisms remain unclear. cPLA(2)α selectively liberates arachidonic acid from membrane phospholipids. Here, we provide the first direct evidence that host 5-lipoxygenase and lipoxygenase products of arachidonic acid, cysteinyl leukotrienes (LTs), contribute to E. coli K1 invasion of HBMEC and penetration into the brain, and their contributions involve protein kinase C alpha (PKCα). These findings demonstrate that arachidonic acid metabolism regulates E. coli penetration of the blood-brain barrier, and studies are needed to further elucidate the mechanisms involved with metabolic products of arachidonic acid for their contribution to E. coli invasion of the blood-brain barrier.
Longkun Zhu; Ravi Maruvada; Adam Sapirstein; Kafait U Malik; Marc Peters-Golden; Kwang Sik Kim
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-08-09
Journal Detail:
Title:  Infection and immunity     Volume:  78     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-22     Completed Date:  2010-11-12     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4302-10     Citation Subset:  IM    
Division of Pediatric Infectious Diseases, Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
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MeSH Terms
Arachidonate 5-Lipoxygenase / metabolism
Arachidonic Acid / metabolism*
Blood-Brain Barrier / microbiology,  physiology*
Brain / blood supply
Endothelial Cells / metabolism*
Escherichia coli / physiology*
Group IV Phospholipases A2 / metabolism
Leukotrienes / metabolism
Mice, Knockout
Protein Kinase C-alpha / metabolism
Grant Support
Reg. No./Substance:
0/Leukotrienes; 506-32-1/Arachidonic Acid; EC 5-Lipoxygenase; EC Kinase C-alpha; EC IV Phospholipases A2

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