Document Detail


Arachidonic acid metabolism by a vitamin D3-differentiated human leukemic cell line.
MedLine Citation:
PMID:  2848399     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
HL-60 is a human promyelocytic cell line that differentiates along the granulocytic pathway when incubated with dimethylsulfoxide and along the monocytic pathway when incubated with 1,25-(OH)2D3. We compared arachidonic acid metabolism in undifferentiated, DMSO-differentiated, and 1,25-(OH)2D3-differentiated cells. DMSO- and 1,25-(OH)2D3-differentiated cells metabolized exogenous arachidonic acid to both cyclo-oxygenase products (predominantly thromboxane B2 and prostaglandin E2) and 5-lipoxygenase products, including leukotriene B4. Undifferentiated cells produce these metabolites in much smaller amounts. DMSO-differentiated cells released a large percentage of phospholipid-bound arachidonic acid in response to stimulation with the ionophore A23187, zymosan, or formylmethionylleucylphenylalanine (FMLP). DMSO-differentiated cells stimulated with A23187 converted released arachidonate to LTB4 and TxB2. In contrast, 1,25-(OH)2D3-differentiated cells released a smaller percentage of phospholipid-bound arachidonate in response to stimuli, and undifferentiated cells released none at all. The three cell types (undifferentiated, DMSO-differentiated, and 1,25-(OH)2D3-differentiated) were homogenized and the 10,000 X g supernatant incubated with [14C]arachidonic acid. The supernatants from the homogenates of the DMSO- and 1,25-(OH)2D3-differentiated cells metabolized [14C]arachidonic acid to cyclooxygenase and lipoxygenase products, but the supernatant from the homogenate of undifferentiated cells did not. These data indicate that differentiation of HL-60 cells with DMSO or 1,25-(OH)2D3 induces cyclooxygenase and 5-lipoxygenase and induces mechanisms for the release of arachidonate from phospholipids by soluble and particulate stimuli.
Authors:
W F Stenson; S L Teitelbaum; Z Bar-Shavit
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research     Volume:  3     ISSN:  0884-0431     ISO Abbreviation:  J. Bone Miner. Res.     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1989-01-05     Completed Date:  1989-01-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8610640     Medline TA:  J Bone Miner Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  561-71     Citation Subset:  IM    
Affiliation:
Department of Medicine, Jewish Hospital, Washington University Medical Center, St. Louis, MO.
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MeSH Terms
Descriptor/Qualifier:
Arachidonic Acid
Arachidonic Acids / metabolism*
Calcimycin / pharmacology
Calcitriol / pharmacology*
Cell Division / drug effects
Cell Fractionation
Dimethyl Sulfoxide
Humans
Leukemia / metabolism*
Leukotriene B4 / biosynthesis
Lipids / analysis
N-Formylmethionine Leucyl-Phenylalanine / pharmacology
Radioimmunoassay
Thromboxane B2 / biosynthesis
Tumor Cells, Cultured
Zymosan / pharmacology
Grant Support
ID/Acronym/Agency:
AR34401/AR/NIAMS NIH HHS; DE05413/DE/NIDCR NIH HHS; DK33165/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Lipids; 32222-06-3/Calcitriol; 506-32-1/Arachidonic Acid; 52665-69-7/Calcimycin; 54397-85-2/Thromboxane B2; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine; 67-68-5/Dimethyl Sulfoxide; 71160-24-2/Leukotriene B4; 9010-72-4/Zymosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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