Document Detail

Arachidonic acid metabolism by nuclei of a retinoic acid--or vitamin D3-differentiated human leukemia cell line HL-60.
MedLine Citation:
PMID:  7938099     Owner:  NLM     Status:  MEDLINE    
Arachidonic acid (AA) metabolism in nuclei of human pro-myelocytic leukemia (HL-60) cells was investigated during retinoic acid (RA)-induced granulocytic differentiation and 1 alpha, 25 dihydroxy-vitamin D3-induced monocytic differentiation. The whole control HL-60 cells and their nuclei hardly converted [1-14C]-AA to any metabolites comigrating with authentic prostaglandins (PGs). On the other hand, RA-treated HL-60 cells acquired the ability to convert [1-14C]-AA to PGE2 predominantly and thromboxane B2 (TXB2) to a small degree, whereas the nuclei of the differentiated cells acquired the ability to convert predominantly to TXB2. In contrast, 1 alpha, 25-dihydroxy-vitamin D3-treated HL-60 cells acquired the ability to convert [1-14C]-AA to PGE2, PGF2 alpha, TXB2 and 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT), whereas the nuclei of the differentiated cells acquired the ability to convert to PGF2 alpha, TXB2 and HHT. The significance of the acquisition of cyclooxygenase and TX synthetase by the nucleus is unclear, but there may be a specific relationship between the specific PGs formed by the nuclear membrane and nuclear events during HL-60 cell differentiation.
K Matsumoto; I Morita; S Murota
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Prostaglandins, leukotrienes, and essential fatty acids     Volume:  51     ISSN:  0952-3278     ISO Abbreviation:  Prostaglandins Leukot. Essent. Fatty Acids     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-11-18     Completed Date:  1994-11-18     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8802730     Medline TA:  Prostaglandins Leukot Essent Fatty Acids     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  51-5     Citation Subset:  IM    
2nd Department of Orthodontics, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
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MeSH Terms
Arachidonic Acid / metabolism*
Calcitriol / pharmacology*
Cell Differentiation / drug effects
Cell Nucleus / drug effects,  metabolism
Dinoprost / biosynthesis
Dinoprostone / biosynthesis
Fatty Acids, Unsaturated / biosynthesis
Granulocytes / drug effects,  metabolism,  pathology
Leukemia, Promyelocytic, Acute / metabolism*,  pathology
Monocytes / drug effects,  metabolism,  pathology
Thromboxane B2 / biosynthesis
Tretinoin / pharmacology*
Tumor Cells, Cultured / drug effects,  metabolism,  pathology
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 302-79-4/Tretinoin; 32222-06-3/Calcitriol; 363-24-6/Dinoprostone; 506-32-1/Arachidonic Acid; 50683-78-8/12-hydroxy-5,8,10-heptadecatrienoic acid; 54397-85-2/Thromboxane B2; 551-11-1/Dinoprost

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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