Document Detail

Arachidonic acid metabolism by bovine placental tissue during the last month of pregnancy.
MedLine Citation:
PMID:  2020749     Owner:  NLM     Status:  MEDLINE    
Conversion of tritiated arachidonic acid (AA) into metabolites of the cyclo- and lipoxygenase pathways by bovine fetal placental tissue (200 mg) and fetal plus maternal placental tissue (400 mg) of Days 255, 265, 275 of gestation and at parturition (n = 5) during a 30 min incubation was measured using reverse-phase high pressure liquid chromatography. Fetal placental tissue produced 13,14-dihydro-15-keto-prostaglandin E2 (PGEM) as the major metabolite, the synthesis of which increased from Day 265 to Day 275 and parturition by 150% and 475%, respectively. In tissues collected at parturition, PGE2 synthesis was also detected. On Day 275 and at parturition fetal placental tissue synthesized the metabolite 12-hydroxyheptadecatrienoic acid (HHT), and throughout the experimental period the lipoxygenase product 15-HETE was detected with synthesis rates increasing over time of gestation. In addition, an unidentified metabolite was regularly found in the radiochromatograms which eluted at 1 h and 1 min (U101), between HHT and 15-HETE. The synthesis of this metabolite decreased as pregnancy progressed. Furthermore, various other polar and nonpolar metabolites pooled under the heading UNID were eluted, the production of which increased over time of gestation. The presence of maternal placental tissue did not influence the synthesis of PGEM, 15-HETE and U101, but the production of HHT was decreased when maternal tissue was present. Also, as pregnancy progressed, maternal placental tissue seemed to contribute to the pool of unidentified metabolites. In conclusion, fetal placental tissue seems to be the major source of the AA metabolites when compared with maternal placental tissue, and AA metabolism by bovine placental tissue is markedly increased throughout the last month of pregnancy, suggesting a role for AA metabolites in mechanisms controlling parturition.
M Hoedemaker; P G Weston; W C Wagner
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Prostaglandins     Volume:  41     ISSN:  0090-6980     ISO Abbreviation:  Prostaglandins     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-05-29     Completed Date:  1991-05-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0320271     Medline TA:  Prostaglandins     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  75-84     Citation Subset:  IM    
Department of Veterinary Biosciences, University of Illinois, Urbana 61801.
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MeSH Terms
Arachidonic Acids / isolation & purification,  metabolism*
Chorionic Villi / metabolism
Chromatography, High Pressure Liquid
Eicosanoids / isolation & purification,  metabolism
Fetus / physiology
Gestational Age
Placenta / metabolism*
Pregnancy, Animal / metabolism*
Reg. No./Substance:
0/Arachidonic Acids; 0/Eicosanoids; 10028-17-8/Tritium

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