Document Detail

Arachidonic acid-induced apoptosis in rat hepatoma AS-30D cells is mediated by reactive oxygen species.
MedLine Citation:
PMID:  19949744     Owner:  NLM     Status:  MEDLINE    
Arachidonic acid at micromolar concentrations produced a drastic increase of the generation of reactive oxygen species (ROS) in rat hepatoma AS-30D cells cultivated in vitro along with an increase in the incidence of apoptotic cell death. Both processes were prevented by trolox, a water-soluble tocopherol derivative, and tempol, a known antioxidative agent. A synthetic hybrid of lipoic acid and trolox or preincubation with N-acetylcysteine were less effective. Preincubation of the cells with etomoxir, a known highly specific irreversible inhibitor of carnitine-palmitoyltransferase I, partly decreased the ROS formation induced by arachidonic acid but it did not affect the increase in apoptosis. Cumulatively, these results indicate that apoptosis induced in hepatoma cells by arachidonic acid is mediated by ROS. They also suggest that this effect is due to arachidonic acid as such and not to its mitochondrial oxidative metabolites.
Dorota Dymkowska; Lech Wojtczak
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Publication Detail:
Type:  Journal Article     Date:  2009-12-01
Journal Detail:
Title:  Acta biochimica Polonica     Volume:  56     ISSN:  1734-154X     ISO Abbreviation:  Acta Biochim. Pol.     Publication Date:  2009  
Date Detail:
Created Date:  2010-01-12     Completed Date:  2010-02-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  14520300R     Medline TA:  Acta Biochim Pol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  711-5     Citation Subset:  IM    
Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland.
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MeSH Terms
Acetylcysteine / pharmacology
Antioxidants / pharmacology
Apoptosis / drug effects*
Arachidonic Acid / pharmacology*,  therapeutic use
Carcinoma, Hepatocellular / drug therapy*
Cell Line, Tumor
Chromans / pharmacology
Cyclic N-Oxides / pharmacology
Epoxy Compounds / pharmacology
Liver Neoplasms / metabolism
Reactive Oxygen Species / metabolism*
Spin Labels
Reg. No./Substance:
0/Antioxidants; 0/Chromans; 0/Cyclic N-Oxides; 0/Epoxy Compounds; 0/Reactive Oxygen Species; 0/Spin Labels; 2226-96-2/tempol; 506-32-1/Arachidonic Acid; 56305-04-5/6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; 616-91-1/Acetylcysteine; 82258-36-4/etomoxir

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