| Arachidonic acid-induced apoptosis in rat hepatoma AS-30D cells is mediated by reactive oxygen species. | |
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MedLine Citation:
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PMID: 19949744 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Arachidonic acid at micromolar concentrations produced a drastic increase of the generation of reactive oxygen species (ROS) in rat hepatoma AS-30D cells cultivated in vitro along with an increase in the incidence of apoptotic cell death. Both processes were prevented by trolox, a water-soluble tocopherol derivative, and tempol, a known antioxidative agent. A synthetic hybrid of lipoic acid and trolox or preincubation with N-acetylcysteine were less effective. Preincubation of the cells with etomoxir, a known highly specific irreversible inhibitor of carnitine-palmitoyltransferase I, partly decreased the ROS formation induced by arachidonic acid but it did not affect the increase in apoptosis. Cumulatively, these results indicate that apoptosis induced in hepatoma cells by arachidonic acid is mediated by ROS. They also suggest that this effect is due to arachidonic acid as such and not to its mitochondrial oxidative metabolites. |
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Authors:
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Dorota Dymkowska; Lech Wojtczak |
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Publication Detail:
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Type: Journal Article Date: 2009-12-01 |
Journal Detail:
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Title: Acta biochimica Polonica Volume: 56 ISSN: 1734-154X ISO Abbreviation: Acta Biochim. Pol. Publication Date: 2009 |
Date Detail:
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Created Date: 2010-01-12 Completed Date: 2010-02-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 14520300R Medline TA: Acta Biochim Pol Country: Poland |
Other Details:
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Languages: eng Pagination: 711-5 Citation Subset: IM |
Affiliation:
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Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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pharmacology Animals Antioxidants / pharmacology Apoptosis / drug effects* Arachidonic Acid / pharmacology*, therapeutic use Carcinoma, Hepatocellular / drug therapy* Cell Line, Tumor Chromans / pharmacology Cyclic N-Oxides / pharmacology Epoxy Compounds / pharmacology Liver Neoplasms / metabolism Rats Reactive Oxygen Species / metabolism* Spin Labels |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Chromans; 0/Cyclic N-Oxides; 0/Epoxy Compounds; 0/Reactive Oxygen Species; 0/Spin Labels; 2226-96-2/tempol; 506-32-1/Arachidonic Acid; 56305-04-5/6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; 616-91-1/Acetylcysteine; 82258-36-4/etomoxir |
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