| Arachidonic acid increases antifungal susceptibility of Candida albicans and Candida dubliniensis. | |
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MedLine Citation:
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PMID: 18971215 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: During Candida albicans infection, arachidonic acid (AA) is released from phospholipids of infected host cell membranes and used by C. albicans as the sole carbon source and for production of eicosanoids. AA can be incorporated into the phospholipids of yeasts, influencing the saturation level and fluidity of yeast cell membranes. It is suggested that the effectiveness of polyene (e.g. amphotericin B) and imidazole (e.g. clotrimazole) antifungals may depend upon the level of unsaturation and ergosterol in the membrane. Therefore, the aim of this study was to evaluate the effect of AA on the cell membrane and susceptibility of C. albicans and Candida dubliniensis biofilms towards amphotericin B and clotrimazole. METHODS: Both yeasts were grown in the presence and absence of AA and the effect of amphotericin B and clotrimazole was examined by confocal laser scanning microscopy, determination of mitochondrial metabolism, unsaturation index of the phospholipid fractions and ergosterol content of the membranes. RESULTS: AA had no effect on the viability of the cells in the biofilm; however, there was an increase in ergosterol levels as well as antifungal susceptibility of biofilms grown in the presence of AA. CONCLUSIONS: AA influences phospholipid unsaturation and ergosterol content of both yeasts C. albicans and C. dublininensis, increasing susceptibility towards the antifungals. Pretreatment of biofilms with polyunsaturated fatty acids may result in the reduction in antifungal dose needed to inhibit biofilms. |
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Authors:
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Ruan Ells; Johan L F Kock; Pieter W J Van Wyk; Piet J Botes; Carolina H Pohl |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-29 |
Journal Detail:
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Title: The Journal of antimicrobial chemotherapy Volume: 63 ISSN: 1460-2091 ISO Abbreviation: J. Antimicrob. Chemother. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-16 Completed Date: 2009-01-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7513617 Medline TA: J Antimicrob Chemother Country: England |
Other Details:
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Languages: eng Pagination: 124-8 Citation Subset: IM |
Affiliation:
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Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amphotericin B
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pharmacology* Antifungal Agents / pharmacology* Arachidonic Acid / pharmacology* Biofilms / drug effects Candida / drug effects*, physiology Cell Membrane / chemistry Clotrimazole / pharmacology* Drug Synergism Ergosterol / analysis Microbial Viability Phospholipids / analysis |
| Chemical | |
Reg. No./Substance:
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0/Antifungal Agents; 0/Phospholipids; 1397-89-3/Amphotericin B; 23593-75-1/Clotrimazole; 506-32-1/Arachidonic Acid; 57-87-4/Ergosterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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