Document Detail

Arachidonic acid depletion extends survival of cold stored platelets by interfering with [Glycoprotein Ibα - 14-3-3ζ] association.
MedLine Citation:
PMID:  22371179     Owner:  NLM     Status:  Publisher    
Introduction. Cold storage of platelets reduces bacterial growth and better preserves their haemostatic properties than current procedures. However, 0°C storage induces [14-3-3ζ-Glycoprotein Ibα] association, 14-3-3ζ release from phospho-Bad, Bad activation and apoptosis. Design and Methods. We investigated whether arachidonic acid, which also binds 14-3-3ζ, contributes to cold-induced apoptosis. Results. Cold storage activated P38-mitogen-activated protein kinase and released arachidonic acid, which accumulated due to cold inactivation of cyclooxygenase-1/thromboxane synthase. Accumulated arachidonic acid released 14-3-3ζ from phospho-Bad and decreased the mitochondrial membrane potential, which are steps in apoptosis induction. Arachidonic acid addition did the same and depletion made platelets resistant against cold-induced apoptosis. Incubation with biotin-arachidonic acid revealed formation of a [arachidonic acid-14-3-3ζ-Glycoprotein Ibα] complex. Indomethacin promoted complex formation by accumulating arachidonic acid and released 14-3-3ζ from cyclooxygenase-1. Arachidonic acid depletion prevented the cold-induced reduction of platelet survival. Conclusions. We conclude that cold storage induced apoptosis through a [arachidonic acid-14-3-3ζ-Glycoprotein Ibα] complex, which released 14-3-3ζ from Bad in an arachidonic acid-dependent manner. Although arachidonic acid depletion reduced agonist-induced thromboxane A2 formation and aggregation, arachidonic acid repletion restored these functions, opening ways to reduce apoptosis during storage without compromising haemostatic functions post-transfusion.
Dianne E van der Wal; Eelo Gitz; Vivian X Du; Kimberly S L Lo; Cornelis A Koekman; Sabine Versteeg; Jan Willem Akkerman
Related Documents :
7118259 - Biomonitoring of occupational toluene exposure.
16997619 - Characterisation of the 1h and 13c nmr spectra of methylcitric acid.
9175509 - The urinary excretion of arsenic metabolites after a single oral administration of dime...
17123469 - The metabolomics of (+/-)-arecoline 1-oxide in the mouse and its formation by human fla...
1006719 - Isolation and identification of a sulfur-containing metabolite of spironolactone from h...
1487329 - Formic acid excretion in comparison with methanol excretion in urine of workers occupat...
9191029 - Influences of intraperitoneally and dietary administered vitamin e and selenium on the ...
9237449 - In vivo imaging of fatty acid incorporation into brain to examine signal transduction a...
23595359 - Enhanced production of pigments by addition of surfactants in submerged fermentation of...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-27
Journal Detail:
Title:  Haematologica     Volume:  -     ISSN:  1592-8721     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417435     Medline TA:  Haematologica     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
The Netherlands.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Targeting intratumoral B-cells with Rituximab in addition to CHOP in angioimmunoblastic T-cell lymph...
Next Document:  Report from the European Myeloma Network on interphase FISH in multiple myeloma and related disorder...