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Arachidonic acid depletion extends survival of cold stored platelets by interfering with [Glycoprotein Ibα - 14-3-3ζ] association.
MedLine Citation:
PMID:  22371179     Owner:  NLM     Status:  Publisher    
Introduction. Cold storage of platelets reduces bacterial growth and better preserves their haemostatic properties than current procedures. However, 0°C storage induces [14-3-3ζ-Glycoprotein Ibα] association, 14-3-3ζ release from phospho-Bad, Bad activation and apoptosis. Design and Methods. We investigated whether arachidonic acid, which also binds 14-3-3ζ, contributes to cold-induced apoptosis. Results. Cold storage activated P38-mitogen-activated protein kinase and released arachidonic acid, which accumulated due to cold inactivation of cyclooxygenase-1/thromboxane synthase. Accumulated arachidonic acid released 14-3-3ζ from phospho-Bad and decreased the mitochondrial membrane potential, which are steps in apoptosis induction. Arachidonic acid addition did the same and depletion made platelets resistant against cold-induced apoptosis. Incubation with biotin-arachidonic acid revealed formation of a [arachidonic acid-14-3-3ζ-Glycoprotein Ibα] complex. Indomethacin promoted complex formation by accumulating arachidonic acid and released 14-3-3ζ from cyclooxygenase-1. Arachidonic acid depletion prevented the cold-induced reduction of platelet survival. Conclusions. We conclude that cold storage induced apoptosis through a [arachidonic acid-14-3-3ζ-Glycoprotein Ibα] complex, which released 14-3-3ζ from Bad in an arachidonic acid-dependent manner. Although arachidonic acid depletion reduced agonist-induced thromboxane A2 formation and aggregation, arachidonic acid repletion restored these functions, opening ways to reduce apoptosis during storage without compromising haemostatic functions post-transfusion.
Dianne E van der Wal; Eelo Gitz; Vivian X Du; Kimberly S L Lo; Cornelis A Koekman; Sabine Versteeg; Jan Willem Akkerman
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-27
Journal Detail:
Title:  Haematologica     Volume:  -     ISSN:  1592-8721     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417435     Medline TA:  Haematologica     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
The Netherlands.
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