Document Detail

Arachidonic acid-containing phosphatidylcholine inhibits lymphocyte proliferation and decreases interleukin-2 and interferon-gamma production from concanavalin A-stimulated rat lymphocytes.
MedLine Citation:
PMID:  11004609     Owner:  NLM     Status:  MEDLINE    
The proliferation of concanavalin A (Con A)-stimulated rat lymphocytes was markedly inhibited by phosphatidylcholine containing arachidonic and stearic acids (PC(A-S)), but not by phosphatidylcholine containing oleic and stearic acids or phosphatidylinositol containing arachidonic and stearic acids. The concentration of PC(A-S) which inhibited Con A-stimulated proliferation by 50% was 31 microM and near total inhibition was observed at 154 microM . Phosphatidylserine containing only oleic acid enhanced proliferation by 37% at a concentration of 31 microM , but phosphatidylethanolamine and phosphatidylcholine containing only oleic acid did not affect proliferation at this concentration. It is concluded that both the head group and the fatty acid composition contribute to the influence of phospholipids on lymphocyte proliferation. The effects of PC(A-S) on T-lymphocyte responses were investigated further. In parallel with the inhibition of proliferation PC(A-S) caused a concentration-dependent decrease in the production of the Th1-type cytokines interleukin (IL)-2 and interferon (IFN)-gamma; inhibition of cytokine production was >85% at the highest concentration of PC(A-S) used (154 microM ). Production of the Th2-type cytokines IL-4 and IL-10 was not affected. The possible role of prostaglandins in mediating the effects of PC(A-S) was examined by adding indomethacin into the medium and the participation of lipid peroxidation was examined by adding vitamin E and vitamin C. Indomethacin and vitamin E did not affect the inhibition caused by PC(A-S) but vitamin C caused a partial reversal. It is concluded that inhibition of T-lymphocyte proliferation by phospholipids involves both the head group and the fatty acyl chains, that this inhibition is not mediated by prostaglandins but may involve some form of oxidant stress and that some phospholipids (e.g., PC(A-S)) can markedly influence cytokine profiles.
A Nishiyama; C R Cavaglieri; R Curi; P C Calder
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1487     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2001-01-26     Completed Date:  2001-06-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  50-60     Citation Subset:  IM    
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 1524, São Paulo, SP, Brazil.
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MeSH Terms
Arachidonic Acid / analysis,  pharmacology*
Ascorbic Acid / pharmacology
Concanavalin A
Indomethacin / pharmacology
Interferon-gamma / biosynthesis*
Interleukin-2 / biosynthesis*
Lipid Peroxidation / drug effects
Lymphocyte Activation / drug effects
Lymphocytes / drug effects*,  immunology,  metabolism
Phosphatidylcholines / chemistry,  pharmacology*
Rats, Wistar
Thymidine / metabolism
Vitamin E / pharmacology
Reg. No./Substance:
0/Interleukin-2; 0/Lipopolysaccharides; 0/Phosphatidylcholines; 10028-17-8/Tritium; 11028-71-0/Concanavalin A; 1406-18-4/Vitamin E; 50-81-7/Ascorbic Acid; 50-89-5/Thymidine; 506-32-1/Arachidonic Acid; 53-86-1/Indomethacin; 82115-62-6/Interferon-gamma

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