Document Detail

Arachidonic acid biosynthesis in non-stimulated and adrenocorticotropin-stimulated Sertoli and Leydig cells.
MedLine Citation:
PMID:  1328799     Owner:  NLM     Status:  MEDLINE    
The biosynthesis of arachidonic acid in Sertoli and Leydig cells isolated from the testes of mature rats has been investigated. Both types of cells incorporated [2-14C]eicosatrienoic acid from the incubation medium and transformed it into arachidonic acid. The administration of adrenocorticotropin (ACTH) to the rats decreased the delta 5 desaturating activity in the isolated testicular cells, while ACTH produced no changes in the uptake of the substrate. Similar results were obtained when ACTH was added to the incubation medium of cells isolated from non-hormone treated rats. The total fatty acid composition of the Sertoli cells isolated from ACTH-treated rats showed a significant increase in the relative percentage of 18:2n-6 and a decrease in the C20 and C22 polyenes. This may indicate that ACTH exerts an inhibitory effect on delta 6, delta 5 and delta 4 desaturase activities. Addition of corticosterone to the incubation medium also produced a significant decrease in arachidonic acid biosynthesis. Because ACTH is known to stimulate the release of corticosterone in vivo, both hormones may act cumulatively in the regulation of arachidonic acid metabolism in Sertoli and Leydig cells.
G E Hurtado de Catalfo; E C Mandon; I N de Gómez Dumm
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  27     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-11-10     Completed Date:  1992-11-10     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  593-8     Citation Subset:  IM    
Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CONICET-UNLP, Facultad de Ciencias Médicas, Argentina.
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MeSH Terms
8,11,14-Eicosatrienoic Acid / metabolism
Adrenocorticotropic Hormone / pharmacology*
Arachidonic Acid / biosynthesis*
Bucladesine / pharmacology
Corticosterone / pharmacology
Fatty Acid Desaturases / metabolism
Fatty Acids / analysis,  metabolism
Leydig Cells / chemistry,  drug effects,  metabolism*
Lipid Metabolism
Lipids / analysis
Rats, Wistar
Sertoli Cells / chemistry,  drug effects,  metabolism*
Reg. No./Substance:
0/Fatty Acids; 0/Lipids; 362-74-3/Bucladesine; 50-22-6/Corticosterone; 506-32-1/Arachidonic Acid; 7324-41-6/8,11,14-Eicosatrienoic Acid; 9002-60-2/Adrenocorticotropic Hormone; EC 1.14.19.-/Fatty Acid Desaturases

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