Document Detail


Arachidonic acid activates NADPH oxidase by a direct, calmodulin-regulated mechanism.
MedLine Citation:
PMID:  9674016     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In intact neutrophils, arachidonic acid rapidly and transiently activated NADPH oxidase-mediated superoxide generation. Inhibitors of protein kinases (staurosporine and H-7) failed to markedly attenuate arachidonic acid-stimulated superoxide generation. Conversely, the calmodulin antagonist W-7 blocked this arachidonic acid-stimulated response. Similarly, diphenylene iodonium potently inhibited superoxide release. These results suggest that arachidonic acid directly activates the membrane-associated NADPH oxidase and calmodulin and/or calmodulin-binding proteins are required for the assembly of the active oxidase.
Authors:
P J Hartfield; J M Robinson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Prostaglandins & other lipid mediators     Volume:  56     ISSN:  1098-8823     ISO Abbreviation:  Prostaglandins Other Lipid Mediat.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-10-01     Completed Date:  1998-10-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9808648     Medline TA:  Prostaglandins Other Lipid Mediat     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-6     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, Neurobiology and Anatomy, Ohio State University, Columbus 43210, USA. P.Hartfield@flinders.edu.au
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MeSH Terms
Descriptor/Qualifier:
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / pharmacology
Animals
Arachidonic Acid / metabolism*
Calcium-Calmodulin-Dependent Protein Kinases / metabolism
Calmodulin / antagonists & inhibitors,  metabolism*
Enzyme Activation
Enzyme Inhibitors / pharmacology
Guinea Pigs
NADPH Oxidase / antagonists & inhibitors,  metabolism*
Neutrophils / enzymology
Nitric Oxide Synthase / antagonists & inhibitors
Onium Compounds / pharmacology
Spectrophotometry
Staurosporine / pharmacology
Sulfonamides / pharmacology
Superoxides / metabolism
Vasodilator Agents / pharmacology
Grant Support
ID/Acronym/Agency:
AI 24321/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Calmodulin; 0/Enzyme Inhibitors; 0/Onium Compounds; 0/Sulfonamides; 0/Vasodilator Agents; 11062-77-4/Superoxides; 244-54-2/diphenyleneiodonium; 506-32-1/Arachidonic Acid; 62996-74-1/Staurosporine; 65595-90-6/W 7; 79458-81-4/N-(6-aminohexyl)-1-naphthalenesulfonamide; 84477-87-2/1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.6.3.1/NADPH Oxidase; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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