| Arachidonate release and prostaglandin production by group IVC phospholipase A2 (cytosolic phospholipase A2gamma). | |
| | |
MedLine Citation:
|
PMID: 12611587 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
While the role of the group IVA Ca(2+)-dependent cytosolic phospholipase A(2)alpha (cPLA(2)alpha) in arachidonic acid (AA) metabolism has been well documented, that of its paralogue, Ca(2+)-independent group IVC PLA(2) (cPLA(2)gamma), has remained uncertain. Here we show, using a transfection strategy, that cPLA(2)gamma has the ability to increase the spontaneous and stimulus-induced release of cellular fatty acids. The AA released by cPLA(2)gamma was metabolized further to prostaglandin E(2) via cyclo-oxygenase-1 (COX-1) in the immediate response, and via COX-2 in the delayed response. Mutation of the putative catalytic-centre residue Ser(82) abrogated the AA-releasing function of cPLA(2)gamma both in vitro and in vivo. Confocal microscopy revealed that cPLA(2)gamma was distributed in the perinuclear endoplasmic reticulum membranes. Mutating the C-terminal prenylation site of cPLA(2)gamma abrogated its intracellular membrane localization and cellular AA-releasing function, without reducing its enzyme activity in vitro. Our results indicate that cPLA(2)gamma is the second cPLA(2) enzyme that contributes to cellular AA metabolism and phospholipid remodelling under appropriate conditions. |
| | |
Authors:
|
Makoto Murakami; Seiko Masuda; Ichiro Kudo |
Related Documents
:
|
7932557 - The synthesis and thymidylate synthase inhibitory activity of l-gamma-l-linked dipeptid... 11553667 - Gamma-hydroxybutyrate modulation of glutamate levels in the hippocampus: an in vivo and... 3391467 - Identification of spinacine as the principal reaction product of gamma-casein with form... 19966457 - A novel catalytic ability of gamma-glutamylcysteine synthetase of escherichia coli and ... 2165927 - Isolation and partial characterization of two glycoproteins from human liver metastases... 20825647 - Multiple sites in the n-terminal half of simian immunodeficiency virus capsid protein c... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Biochemical journal Volume: 372 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 2003 Jun |
Date Detail:
|
Created Date: 2003-06-04 Completed Date: 2003-07-23 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2984726R Medline TA: Biochem J Country: England |
Other Details:
|
Languages: eng Pagination: 695-702 Citation Subset: IM |
Affiliation:
|
Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. mako@pharm.showa-u.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Substitution Animals Arachidonic Acid / metabolism* Arachidonic Acids / pharmacology Binding Sites Blotting, Northern Cells, Cultured Cyclooxygenase 1 Cyclooxygenase 2 Cytosol / enzymology DNA, Complementary / genetics Endoplasmic Reticulum / metabolism Humans Isoenzymes / antagonists & inhibitors, chemistry, metabolism Membrane Proteins Mice Microscopy, Confocal Nitrobenzenes / pharmacology Phospholipases A / antagonists & inhibitors, chemistry, genetics, metabolism* Phospholipases A2 Phosphonic Acids / pharmacology Prostaglandin-Endoperoxide Synthases / chemistry, metabolism Prostaglandins / biosynthesis* Sulfonamides / pharmacology Transfection / methods |
| Chemical | |
Reg. No./Substance:
|
0/Arachidonic Acids; 0/DNA, Complementary; 0/Isoenzymes; 0/Membrane Proteins; 0/Nitrobenzenes; 0/Phosphonic Acids; 0/Prostaglandins; 0/Sulfonamides; 0/methyl arachidonylfluorophosphonate; 123653-11-2/N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; 506-32-1/Arachidonic Acid; EC 1.14.99.1/Cyclooxygenase 1; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS1 protein, human; EC 1.14.99.1/PTGS2 protein, human; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases; EC 1.14.99.1/Ptgs1 protein, mouse; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Carbonic anhydrase-related protein is a novel binding protein for inositol 1,4,5-trisphosphate recep...
Next Document: Leucine-zipper-mediated homo- and hetero-dimerization of GIT family p95-ARF GTPase-activating protei...