| Arachidonate 5-lipoxygenase and its new inhibitors. | |
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MedLine Citation:
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PMID: 6432877 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The 5-lipoxygenases of guinea pig peritoneal polymorphonuclear leukocytes and of rat basophilic leukemia cells have been solubilized, purified partially by affinity chromatography, and shown to convert arachidonic acid principally to 5-hydroperoxy-6,8,11,14- eicosatetraenoic acid. The activity of both 5-lipoxygenases is calcium dependent and enhanced by adenosine triphosphate and other nucleotides in the presence of calcium ion. Both 5-lipoxygenase activity and leukotriene generation by sensitized guinea pig lung tissue challenged with antigen were suppressed substantially by specific benzoquinone and flavonoid inhibitors. The in vivo significance of the findings will be explored with potent and selective lipoxygenase inhibitors, which are delineated in the 5-lipoxygenase model systems. |
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Authors:
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S Yamamoto; T Yoshimoto; M Furukawa; T Horie; S Watanabe-Kohno |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of allergy and clinical immunology Volume: 74 ISSN: 0091-6749 ISO Abbreviation: J. Allergy Clin. Immunol. Publication Date: 1984 Sep |
Date Detail:
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Created Date: 1984-10-24 Completed Date: 1984-10-24 Revised Date: 2010-05-07 |
Medline Journal Info:
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Nlm Unique ID: 1275002 Medline TA: J Allergy Clin Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 349-52 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonate Lipoxygenases Basophils / enzymology Benzoquinones* Cell Line Flavones* Flavonoids / pharmacology* Guinea Pigs Kinetics Leukemia, Experimental / enzymology* Lipoxygenase / isolation & purification Lipoxygenase Inhibitors* Lung / enzymology Muscle Contraction / drug effects Neutrophils / enzymology* Quinones / pharmacology* Rats SRS-A / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Benzoquinones; 0/Flavones; 0/Flavonoids; 0/Lipoxygenase Inhibitors; 0/Quinones; 0/SRS-A; 34334-69-5/cirsiliol; 80809-81-0/2,3,5-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone; EC 1.13.11.-/Arachidonate Lipoxygenases; EC 1.13.11.12/Lipoxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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