| Arabinosylated lipoarabinomannan-mediated protection in visceral leishmaniasis through up-regulation of toll-like receptor 2 signaling: an immunoprophylactic approach. | |
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MedLine Citation:
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PMID: 20500089 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Visceral leishmaniasis is characterized by severe immunosuppression of the host cell, resulting in loss of the proinflammatory response. Toll-like receptor 2 (TLR2) is involved in myriad disease forms, including visceral leishmaniasis. During Leishmania donovani infection, the parasite modulates TLR2 to suppress interleukin 12 production, indicating the possible involvement of TLR2 in regulation of the immune response against L. donovani infection. Arabinosylated lipoarabinomannan (Ara-LAM) possesses immunomodulatory properties and induces proinflammatory responses via induction of TLR2-mediated signaling. Here, we found that pretreatment of L. donovani-infected macrophages with Ara-LAM caused a significant increase in TLR2 expression along with the activation of TLR2-mediated downstream signaling, facilitating active nuclear translocation of nuclear factor kappaB. These events culminated in up-regulation of the proinflammatory response, which was abrogated by treatment with TLR2-specific small interfering RNA. In vivo experiments were also suggestive of Ara-LAM playing a long-term protective role. This study demonstrates that Ara-LAM confers protection against leishmanial pathogenesis via TLR2 signaling-mediated induction of the proinflammatory response. |
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Authors:
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Parna Bhattacharya; Surajit Bhattacharjee; Gaurav Gupta; Saikat Majumder; Anupam Adhikari; Asok Mukherjee; Suchandra Bhattacharyya Majumdar; Bhaskar Saha; Subrata Majumdar |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of infectious diseases Volume: 202 ISSN: 1537-6613 ISO Abbreviation: J. Infect. Dis. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-03 Completed Date: 2010-06-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: United States |
Other Details:
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Languages: eng Pagination: 145-55 Citation Subset: AIM; IM |
Affiliation:
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Division of Molecular Medicine, Bose Institute, Kolkata -700054, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Female Immunologic Factors / immunology Leishmaniasis, Visceral / immunology*, metabolism Lipopolysaccharides / chemistry, immunology* Macrophages, Peritoneal / metabolism, parasitology Male Mice Mice, Inbred BALB C Mice, Inbred C57BL RNA Interference RNA, Small Interfering Signal Transduction / immunology* Toll-Like Receptor 2 / genetics, metabolism* Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Immunologic Factors; 0/Lipopolysaccharides; 0/RNA, Small Interfering; 0/Tlr2 protein, mouse; 0/Toll-Like Receptor 2; 0/lipoarabinomannan |
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