| Arabinogalactans and arabinogalactan-proteins induce embryogenesis in wheat (Triticum aestivum L.) microspore culture. | |
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MedLine Citation:
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PMID: 16133349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to improve induction of embryogenesis in wheat microspore culture in order to obtain a high number of regenerable embryos. The arabinogalactan (AG) Larcoll and the arabinogalactan-protein (AGP) from gum arabic were tested on two spring genotypes to see if they could increase microspore viability and induce embryogenesis in the microspore culture. Adding Larcoll significantly decreased microspore mortality in both genotypes regardless of the presence or absence of ovaries in the culture. Similarly, gum arabic had a strong effect on the number of embryos produced and regenerated green plants. In fact, by using only gum arabic we were able to obtain green plants from wheat microspore cultures without the presence of ovaries. In addition to preventing a high mortality rate of the cells, our results show that the induction of embryogenesis in wheat microspore cultures is strongly affected by the use of both AG or AGP. |
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Authors:
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Jocelyne Letarte; Ecaterina Simion; Mai Miner; Ken J Kasha |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-08-23 |
Journal Detail:
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Title: Plant cell reports Volume: 24 ISSN: 0721-7714 ISO Abbreviation: Plant Cell Rep. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-01-06 Completed Date: 2006-05-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9880970 Medline TA: Plant Cell Rep Country: Germany |
Other Details:
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Languages: eng Pagination: 691-8 Citation Subset: IM |
Affiliation:
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Department of Plant Agriculture, University of Guelph, Ontario, Canada, N1G 2W1. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Culture Media Galactans / metabolism* Mucoproteins / physiology* Plant Proteins / physiology Seeds / growth & development* Triticum / embryology* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Galactans; 0/Mucoproteins; 0/Plant Proteins; 0/arabinogalactan proteins; 9036-66-2/arabinogalactan |
| Comments/Corrections | |
Erratum In:
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Plant Cell Rep. 2006 Aug;25(8):877 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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