Document Detail


Arabinogalactan for hepatic drug delivery.
MedLine Citation:
PMID:  7533005     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Arabinogalactan, a polysaccharide from the tree Larix occidentalis, has been purified and its biological and physical properties described. Intravenous injection of radiolabeled arabinogalactan (4 mg/kg) in rats resulted in 52.5% of the dose being present in the liver, while prior injection of asialofetuin (100 mg/kg) reduced hepatic radioactivity to 3.54%. Gel chromatography indicates arabinogalactan is a single species of 19 kDa, while light scattering gave a molecular weight of 40 kDa. Glycosyl linkage analysis of arabinogalactan is consistent with a highly branched structure comprising a backbone of 1,3-linked galactopyranose connected by 1,3-glycosidic linkages, comprised of 3,4,6-,3,6-, and 3,4- as well as 3-linked residues. In the carbon-13 NMR spectra, the major resonances of arabinogalactan are assigned to beta-galactopyranose, beta-arabinofuranose, and beta-arabinopyranose. Arabinogalactan produced no adverse reactions in single intravenous dose (mouse, 5000 mg/kg) and repeat dose toxicity studies (rats, 500 mg/kg/day, 90 days). When tritiated arabinogalactan was injected, radioactivity cleared from the liver with a half-life of 3.42 days. Arabinogalactan has properties that make it suitable as a carrier for delivering diagnostic or therapeutic agents to hepatocytes via the asialoglycoprotein receptor.
Authors:
E V Groman; P M Enriquez; C Jung; L Josephson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Bioconjugate chemistry     Volume:  5     ISSN:  1043-1802     ISO Abbreviation:  Bioconjug. Chem.     Publication Date:    1994 Nov-Dec
Date Detail:
Created Date:  1995-04-04     Completed Date:  1995-04-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9010319     Medline TA:  Bioconjug Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  547-56     Citation Subset:  IM    
Affiliation:
Advanced Magnetics, Inc., Cambridge, Massachusetts 02138.
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MeSH Terms
Descriptor/Qualifier:
Animals
Asialoglycoprotein Receptor
Asialoglycoproteins / pharmacology
Carbohydrate Conformation
Cobalt Radioisotopes
Drug Carriers*
Drug Stability
Galactans / chemistry,  pharmacokinetics*,  toxicity
Injections, Intravenous
Liver / metabolism*
Magnetic Resonance Spectroscopy
Male
Metabolic Clearance Rate
Molecular Weight
Rats
Receptors, Cell Surface / metabolism
Tissue Distribution
alpha-Fetoproteins / pharmacology
Chemical
Reg. No./Substance:
0/Asialoglycoprotein Receptor; 0/Asialoglycoproteins; 0/Cobalt Radioisotopes; 0/Drug Carriers; 0/Galactans; 0/Receptors, Cell Surface; 0/alpha-Fetoproteins; 0/asialofetuin; 9036-66-2/arabinogalactan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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