| Arabinogalactan for hepatic drug delivery. | |
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MedLine Citation:
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PMID: 7533005 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Arabinogalactan, a polysaccharide from the tree Larix occidentalis, has been purified and its biological and physical properties described. Intravenous injection of radiolabeled arabinogalactan (4 mg/kg) in rats resulted in 52.5% of the dose being present in the liver, while prior injection of asialofetuin (100 mg/kg) reduced hepatic radioactivity to 3.54%. Gel chromatography indicates arabinogalactan is a single species of 19 kDa, while light scattering gave a molecular weight of 40 kDa. Glycosyl linkage analysis of arabinogalactan is consistent with a highly branched structure comprising a backbone of 1,3-linked galactopyranose connected by 1,3-glycosidic linkages, comprised of 3,4,6-,3,6-, and 3,4- as well as 3-linked residues. In the carbon-13 NMR spectra, the major resonances of arabinogalactan are assigned to beta-galactopyranose, beta-arabinofuranose, and beta-arabinopyranose. Arabinogalactan produced no adverse reactions in single intravenous dose (mouse, 5000 mg/kg) and repeat dose toxicity studies (rats, 500 mg/kg/day, 90 days). When tritiated arabinogalactan was injected, radioactivity cleared from the liver with a half-life of 3.42 days. Arabinogalactan has properties that make it suitable as a carrier for delivering diagnostic or therapeutic agents to hepatocytes via the asialoglycoprotein receptor. |
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Authors:
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E V Groman; P M Enriquez; C Jung; L Josephson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Bioconjugate chemistry Volume: 5 ISSN: 1043-1802 ISO Abbreviation: Bioconjug. Chem. Publication Date: 1994 Nov-Dec |
Date Detail:
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Created Date: 1995-04-04 Completed Date: 1995-04-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 547-56 Citation Subset: IM |
Affiliation:
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Advanced Magnetics, Inc., Cambridge, Massachusetts 02138. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Asialoglycoprotein Receptor Asialoglycoproteins / pharmacology Carbohydrate Conformation Cobalt Radioisotopes Drug Carriers* Drug Stability Galactans / chemistry, pharmacokinetics*, toxicity Injections, Intravenous Liver / metabolism* Magnetic Resonance Spectroscopy Male Metabolic Clearance Rate Molecular Weight Rats Receptors, Cell Surface / metabolism Tissue Distribution alpha-Fetoproteins / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Asialoglycoprotein Receptor; 0/Asialoglycoproteins; 0/Cobalt Radioisotopes; 0/Drug Carriers; 0/Galactans; 0/Receptors, Cell Surface; 0/alpha-Fetoproteins; 0/asialofetuin; 9036-66-2/arabinogalactan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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