Document Detail


Arabidopsis vacuolar H-ATPase subunit E isoform 1 is required for Golgi organization and vacuole function in embryogenesis.
MedLine Citation:
PMID:  15610355     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vacuolar H(+)-ATPases play an important role in maintaining the pH of endomembrane compartments in eukaryotic cells. The functional relevance of this homeostasis for multicellular development has not been studied in plants. Here, we analyze the biological consequences resulting from the lack of subunit E isoform 1 (VHA-E1) encoded by the Arabidopsis TUFF gene. tuff mutant embryos are lethal, displaying variably enlarged cells with multiple nuclei, large vacuoles containing inclusions, abnormal organization of Golgi stacks, and cell wall defects. Rescue of embryo lethality by cell cycle-regulated expression of VHA-E1 results in abnormal seedlings with non-functional meristems and defective cell differentiation. VHA-E1 is the predominant isoform in embryogenesis whereas VHA-E3 is expressed mainly in the endosperm and surrounding maternal tissues during seed development, and VHA-E2 is pollen-specific. VHA-E1 protein accumulates at endomembrane compartments including vacuoles and endosomes, but appears absent from the plasma membrane. Our results suggest an essential role for VHA-E1 in maintaining a functional secretory system during somatic development but not in the haploid gametophytes.
Authors:
Georg Strompen; Jan Dettmer; York-Dieter Stierhof; Karin Schumacher; Gerd Jürgens; Ulrike Mayer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  41     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-21     Completed Date:  2005-08-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  125-32     Citation Subset:  IM    
Affiliation:
ZMBP, Entwicklungsgenetik, Universität Tübingen, Auf der Morgenstelle 3, D-72076 Tübingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Arabidopsis / embryology,  enzymology*,  genetics
Arabidopsis Proteins
Chromosome Mapping
Embryonic Development*
Golgi Apparatus / enzymology,  genetics*
Hydrogen-Ion Concentration
Isoenzymes
Molecular Sequence Data
Mutation
Phenotype
Recombinant Fusion Proteins / genetics,  metabolism
Seeds / enzymology,  genetics,  ultrastructure
Sequence Alignment
Vacuolar Proton-Translocating ATPases / genetics*,  metabolism
Vacuoles / enzymology,  genetics*
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Isoenzymes; 0/Recombinant Fusion Proteins; 0/TUFF protein, Arabidopsis; EC 3.6.1.-/Vacuolar Proton-Translocating ATPases

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