Document Detail

Arabidopsis transient expression analysis reveals that activation of GLABRA2 may require concurrent binding of GLABRA1 and GLABRA3 to the promoter of GLABRA2.
MedLine Citation:
PMID:  18948276     Owner:  NLM     Status:  MEDLINE    
Transcription factors regulate gene expression by directly binding the cis-acting regulatory elements of target genes via their DNA-binding domains or by interacting with other transcription factors. Trichome cell fate determination in Arabidopsis utilizes a lateral inhibition mechanism that relies on the interplay of transcription factors. GLABRA1 (GL1), an R2R3 MYB transcription factor, GLABRA3 (GL3), a basic helix-loop-helix (bHLH) transcription factor, and TRANSPARENT TESTA GLABRA1 (TTG1), a WD40 protein, are believed to form a transcriptional activator complex to control the transcription of GLABRA2 (GL2), which in turn induces trichome formation in shoots. However, the molecular mechanism of the regulation of GL2 expression by this activator complex is still poorly understood. Here we report that GL1 and GL3 control GL2 expression by a previously unrecognized mechanism in which in addition to the protein-protein interaction between GL1 and GL3, concurrent binding of GL1 and GL3 to the promoter of GL2 via their own DNA-binding domains is probably required to activate GL2. We demonstrate that disruption or deletion of the DNA-binding domains in either GL1 or GL3 completely abolishes the transcriptional activity of the GL1-GL3 complex in activating GL2. These results provide new insight into the interplay of GL1 and GL3 transcription factors in the activation of GL2.
Shucai Wang; Jin-Gui Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-22
Journal Detail:
Title:  Plant & cell physiology     Volume:  49     ISSN:  1471-9053     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-08     Completed Date:  2009-01-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1792-804     Citation Subset:  IM    
Department of Botany, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
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MeSH Terms
Arabidopsis / genetics*,  metabolism
Arabidopsis Proteins / genetics,  metabolism*
Basic Helix-Loop-Helix Transcription Factors / genetics,  metabolism*
DNA-Binding Proteins / genetics,  metabolism*
Gene Expression Regulation, Plant
Homeodomain Proteins / genetics,  metabolism*
Plants, Genetically Modified / genetics,  metabolism
Promoter Regions, Genetic
Protein Interaction Domains and Motifs
RNA, Plant / genetics
Transcriptional Activation
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Basic Helix-Loop-Helix Transcription Factors; 0/DNA-Binding Proteins; 0/GL2 protein, Arabidopsis; 0/GL3 protein, Arabidopsis; 0/Homeodomain Proteins; 0/RNA, Plant; 0/TTG1 protein, Arabidopsis; 144417-31-2/GL1 protein, Arabidopsis

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