Document Detail

The Arabidopsis thaliana sulfiredoxin is a plastidic cysteine-sulfinic acid reductase involved in the photooxidative stress response.
MedLine Citation:
PMID:  17217469     Owner:  NLM     Status:  MEDLINE    
The 2-cysteine peroxiredoxins (2-Cys-Prxs) are antioxidants that reduce peroxides through a thiol-based mechanism. During catalysis, these ubiquitous enzymes are occasionally inactivated by the substrate-dependent oxidation of the catalytic cysteine to the sulfinic acid (-SO2H) form, and are reactivated by reduction by sulfiredoxin (Srx), an enzyme recently identified in yeast and in mammal cells. In plants, 2-Cys-Prxs constitute the most abundant Prxs and are located in chloroplasts. Here we have characterized the unique Srx gene in Arabidopsis thaliana (AtSrx) from a functional point of view, and analyzed the phenotype of two AtSrx knockout (AtSrx-) mutant lines. AtSrx is a chloroplastic enzyme displaying sulfinic acid reductase activity, as shown by the ability of the recombinant AtSrx to reduce the overoxidized 2-Cys-Prx form in vitro, and by the accumulation of the overoxidized Prx in mutant lines lacking Srx in vivo. Furthermore, AtSrx mutants exhibit an increased tolerance to photooxidative stress generated by high light combined with low temperature. These data establish that, as in yeast and in mammals, plant 2-Cys-Prxs are subject to substrate-mediated inactivation reversed by Srx, and suggest that the 2-Cys-Prx redox status and sulfiredoxin are parts of a signaling mechanism participating in plant responses to oxidative stress.
Pascal Rey; Noëlle Bécuwe; Marie-Bénédicte Barrault; Dominique Rumeau; Michel Havaux; Benoît Biteau; Michel B Toledano
Related Documents :
812869 - Magnesium and anion requirements of rodb mutants of bacillus subtilis.
16597989 - Identification of salmonella enterica serovar typhimurium genes important for survival ...
2111169 - Determinants of visual pigment absorbance: role of charged amino acids in the putative ...
16245349 - Thermostabilization of porcine kidney d-amino acid oxidase by a single amino acid subst...
3800869 - Comparison of growth, exogenous auxin sensitivity, and endogenous indole-3-acetic acid ...
16260759 - Evolution of an acylase active on cephalosporin c.
15590999 - The role of the carnitine system in human metabolism.
10674359 - Dietary trans fatty acids affect docosahexaenoic acid concentrations in plasma and live...
1233309 - Fatty acid pattern of cerebral lipids in cyanide encephalopathy.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-01
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  49     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-24     Completed Date:  2007-06-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  505-14     Citation Subset:  IM    
CEA, DSV, DEVM, LEMP, Laboratoire d'Ecophysiologie Moléculaire des Plantes, UMR 6191 CNRS-CEA-Université de la Méditerranée, 13108 Saint-Paul-lez-Durance, Cedex, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adaptation, Physiological
Amino Acid Sequence
Arabidopsis / enzymology*
Arabidopsis Proteins / metabolism*
Chloroplasts / enzymology*
Gene Expression
Molecular Sequence Data
Mutagenesis, Insertional
Oxidoreductases Acting on Sulfur Group Donors / metabolism*
Peroxidases / metabolism*
Sequence Homology, Amino Acid
Reg. No./Substance:
0/Arabidopsis Proteins; EC 1.11.1.-/Peroxidases; EC; EC 1.8.-/Oxidoreductases Acting on Sulfur Group Donors; EC protein, Arabidopsis

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  HY5 is a point of convergence between cryptochrome and cytokinin signalling pathways in Arabidopsis ...
Next Document:  The phenotype of soluble starch synthase IV defective mutants of Arabidopsis thaliana suggests a nov...