Document Detail


The Arabidopsis thaliana Myo-inositol 1-phosphate synthase1 gene is required for Myo-inositol synthesis and suppression of cell death.
MedLine Citation:
PMID:  20215587     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
l-myo-inositol 1-phosphate synthase (MIPS; EC 5.5.1.4) catalyzes the rate-limiting step in the synthesis of myo-inositol, a critical compound in the cell. Plants contain multiple MIPS genes, which encode highly similar enzymes. We characterized the expression patterns of the three MIPS genes in Arabidopsis thaliana and found that MIPS1 is expressed in most cell types and developmental stages, while MIPS2 and MIPS3 are mainly restricted to vascular or related tissues. MIPS1, but not MIPS2 or MIPS3, is required for seed development, for physiological responses to salt and abscisic acid, and to suppress cell death. Specifically, a loss in MIPS1 resulted in smaller plants with curly leaves and spontaneous production of lesions. The mips1 mutants have lower myo-inositol, ascorbic acid, and phosphatidylinositol levels, while basal levels of inositol (1,4,5)P(3) are not altered in mips1 mutants. Furthermore, mips1 mutants exhibited elevated levels of ceramides, sphingolipid precursors associated with cell death, and were complemented by a MIPS1-green fluorescent protein (GFP) fusion construct. MIPS1-, MIPS2-, and MIPS3-GFP each localized to the cytoplasm. Thus, MIPS1 has a significant impact on myo-inositol levels that is critical for maintaining levels of ascorbic acid, phosphatidylinositol, and ceramides that regulate growth, development, and cell death.
Authors:
Janet L Donahue; Shannon R Alford; Javad Torabinejad; Rachel E Kerwin; Aida Nourbakhsh; W Keith Ray; Marcy Hernick; Xinyi Huang; Blair M Lyons; Pyae P Hein; Glenda E Gillaspy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-03-09
Journal Detail:
Title:  The Plant cell     Volume:  22     ISSN:  1532-298X     ISO Abbreviation:  Plant Cell     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-26     Completed Date:  2010-07-14     Revised Date:  2011-07-25    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  888-903     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Virginia Tech, Blacksburg, Virginia 24061, USA.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / enzymology,  genetics*
Arabidopsis Proteins / genetics,  metabolism*
Ascorbic Acid / metabolism
Cell Death*
Ceramides / metabolism
DNA, Plant / genetics
Gene Expression Regulation, Plant
Inositol / biosynthesis*
Mutagenesis, Insertional
Mutation
Myo-Inositol-1-Phosphate Synthase / genetics*,  metabolism
Phosphatidylinositols / metabolism
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Ceramides; 0/DNA, Plant; 0/Phosphatidylinositols; 50-81-7/Ascorbic Acid; 6917-35-7/Inositol; EC 5.5.1.4/Myo-Inositol-1-Phosphate Synthase
Comments/Corrections
Comment In:
Plant Cell. 2010 Mar;22(3):537   [PMID:  20233949 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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