Document Detail


Arabidopsis suppressor mutant of abh1 shows a new face of the already known players: ABH1 (CBP80) and ABI4-in response to ABA and abiotic stresses during seed germination.
MedLine Citation:
PMID:  23196831     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although the importance of abscisic acid (ABA) in plant development and response to abiotic and biotic stresses is well recognized, the molecular basis of the signaling pathway has not been fully elucidated. Mutants in genes related to ABA are widely used as a tool for gaining insight into the mechanisms of ABA signal transduction and ABA-dependent stress response. We used a genetic approach of a suppressor screening in order to decipher the interaction between ABH1 (CBP80) and other components of ABA signaling. ABH1 (CBP80) encodes a large subunit of CBC (CAP BINDING COMPLEX) and the abh1 mutant is drought-tolerant and hypersensitive to ABA during seed germination. The suppressor mutants of abh1 were generated after chemical mutagenesis. The mutant named soa1 (suppressor of abh1 hypersensitivity to ABA 1) displayed an ABA-insensitive phenotype during seed germination. The genetic analysis showed that the soa1 phenotype is dominant in relation to abh1 and segregates as a single locus. Based on soa1's response to a wide spectrum of physiological assays during different stages of development, we used the candidate-genes approach in order to identify a suppressor gene. The molecular analysis revealed that mutation causing the phenotype of soa1 occurred in the ABI4 (ABA insensitive 4) gene. Analysis of pre-miR159 expression, whose processing depends on CBC, as well as targets of miR159: MYB33 and MYB101, which are positive regulators of ABA signaling, revealed a possible link between CBP80 (ABH1) and ABI4 presented here.
Authors:
Agata Daszkowska-Golec; Weronika Wojnar; Marta Rosikiewicz; Iwona Szarejko; Miroslaw Maluszynski; Zofia Szweykowska-Kulinska; Artur Jarmolowski
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-30
Journal Detail:
Title:  Plant molecular biology     Volume:  81     ISSN:  1573-5028     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-24     Completed Date:  2013-02-20     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  189-209     Citation Subset:  IM    
Affiliation:
Department of Genetics, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland. agata.daszkowska@us.edu.pl
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MeSH Terms
Descriptor/Qualifier:
Abscisic Acid / pharmacology
Arabidopsis / drug effects,  genetics*,  growth & development,  physiology
Arabidopsis Proteins / genetics*
Droughts
Epistasis, Genetic
Genes, Plant
Genes, Suppressor
Germination / genetics
Mutation
Osmotic Pressure
RNA Cap-Binding Proteins / genetics*
RNA-Binding Proteins / genetics*
Seeds / growth & development
Stress, Physiological
Transcription Factors / genetics*
Chemical
Reg. No./Substance:
0/ABH1 protein, Arabidopsis; 0/ABI4 protein, Arabidopsis; 0/Arabidopsis Proteins; 0/CBP80 protein, Arabidopsis; 0/RNA Cap-Binding Proteins; 0/RNA-Binding Proteins; 0/Transcription Factors; 21293-29-8/Abscisic Acid
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