Document Detail


Arabidopsis small RNAs and their targets during cyst nematode parasitism.
MedLine Citation:
PMID:  18986258     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plant-parasitic cyst nematodes induce the formation of specialized feeding cells in infected roots, which involves plant developmental processes that have been shown to be influenced by microRNAs (miRNAs) and other small RNAs. This observation provided the foundation to investigate the potential involvement of small RNAs in plant-cyst nematode interactions. First, we examined the susceptibilities of Arabidopsis DICER-like (dcl) and RNA-dependent RNA polymerase (rdr) mutants to the sugar beet cyst nematode Heterodera schachtii. The examined mutants exhibited a trend of decreased susceptibility, suggesting a role of small RNAs mediating gene regulation processes during the plant-nematode interaction. Second, we generated two small RNA libraries from aseptic Arabidopsis roots harvested at 4 and 7 days after infection with surface-sterilized H. schachtii. Sequences of known miRNAs as well as novel small interfering (si)RNAs were identified. Following this discovery, we used real-time reverse-transcriptase polymerase chain reaction to quantify a total of 15 Arabidopsis transcripts that are known targets of six of the different miRNA families found in our study (miR160, miR164, miR167, miR171, miR396, and miR398) in inoculated and noninoculated Arabidopsis roots. Our analyses showed mostly negative correlations between miRNA accumulation and target gene mRNA abundance, suggesting regulatory roles of these miRNAs during parasitism. Also, we identified a total of 125 non-miRNA siRNAs. Some of these siRNAs perfectly complement protein-coding mRNAs or match transposon or retrotransposon sequences in sense or antisense orientations. We further quantified a group of siRNAs in H. schachtii-inoculated roots. The examined siRNAs exhibited distinct expression patterns in infected and noninfected roots, providing additional evidence for the implication of small RNAs in cyst nematode parasitism. These data lay the foundation for detailed analyses of the functions of small RNAs during phytonematode parasitism.
Authors:
Tarek Hewezi; Peter Howe; Tom R Maier; Thomas J Baum
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular plant-microbe interactions : MPMI     Volume:  21     ISSN:  0894-0282     ISO Abbreviation:  Mol. Plant Microbe Interact.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-06     Completed Date:  2008-12-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107902     Medline TA:  Mol Plant Microbe Interact     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1622-34     Citation Subset:  IM    
Affiliation:
Department of Plant Pathology, Iowa State University, Ames 50011, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arabidopsis / genetics*,  metabolism,  parasitology*
Arabidopsis Proteins / genetics
Cell Cycle Proteins / genetics
Cloning, Molecular
Gene Expression Regulation, Plant
Gene Library
MicroRNAs / genetics*
Mutation
Plant Roots / genetics,  metabolism,  parasitology
RNA Replicase / genetics
RNA, Plant / genetics
RNA, Small Interfering / genetics
Reverse Transcriptase Polymerase Chain Reaction
Ribonuclease III / genetics
Sequence Analysis, RNA
Tylenchoidea / pathogenicity*
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Cell Cycle Proteins; 0/MicroRNAs; 0/RNA, Plant; 0/RNA, Small Interfering; EC 2.7.7.48/RNA Replicase; EC 3.1.26.3/DCL1 protein, Arabidopsis; EC 3.1.26.3/Ribonuclease III

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