Document Detail


The Arabidopsis small G protein ROP2 is activated by light in guard cells and inhibits light-induced stomatal opening.
MedLine Citation:
PMID:  18178769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
ROP small G proteins function as molecular switches in diverse signaling processes. Here, we investigated signals that activate ROP2 in guard cells. In guard cells of Vicia faba expressing Arabidopsis thaliana constitutively active (CA) ROP2 fused to red fluorescent protein (RFP-CA-ROP2), fluorescence localized exclusively at the plasma membrane, whereas a dominant negative version of RFP-ROP2 (DN-ROP2) localized in the cytoplasm. In guard cells expressing green fluorescent protein-ROP2, the relative fluorescence intensity at the plasma membrane increased upon illumination, suggesting that light activates ROP2. Unlike previously reported light-activated factors, light-activated ROP2 inhibits rather than accelerates light-induced stomatal opening; stomata bordered by guard cells transformed with CA-rop2 opened less than controls upon light irradiation. When introduced into guard cells together with CA-ROP2, At RhoGDI1, which encodes a guanine nucleotide dissociation inhibitor, inhibited plasma membrane localization of CA-ROP2 and abolished the inhibitory effect of CA-ROP2 on light-induced stomatal opening, supporting the negative effect of active ROP2 on stomatal opening. Mutant rop2 Arabidopsis guard cells showed phenotypes similar to those of transformed V. faba guard cells; CA-rop2 stomata opened more slowly and to a lesser extent, and DN-rop2 stomata opened faster than wild-type stomata in response to light. Moreover, in rop2 knockout plants, stomata opened faster and to a greater extent than wild-type stomata in response to light. Thus, ROP2 is a light-activated negative factor that attenuates the extent of light-induced changes in stomatal aperture. The inhibition of light-induced stomatal opening by light-activated ROP2 suggests the existence of feedback regulatory mechanisms through which stomatal apertures may be finely controlled.
Authors:
Byeong Wook Jeon; Jae-Ung Hwang; Youngkyu Hwang; Won-Yong Song; Ying Fu; Ying Gu; Fang Bao; Daeshik Cho; June M Kwak; Zhenbiao Yang; Youngsook Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-01-04
Journal Detail:
Title:  The Plant cell     Volume:  20     ISSN:  1040-4651     ISO Abbreviation:  Plant Cell     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-03-03     Completed Date:  2008-05-15     Revised Date:  2010-09-22    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  75-87     Citation Subset:  IM    
Affiliation:
POSTECH-UZH Global Research Laboratories, Division of Molecular Life Sciences, POSTECH, Pohang 790-784, Korea.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / enzymology*,  genetics,  radiation effects
Arabidopsis Proteins / genetics,  metabolism*
Cell Membrane / enzymology,  radiation effects
Enzyme Activation / radiation effects
GTP-Binding Proteins / genetics,  metabolism*
Gene Expression Regulation, Plant
Light*
Mutant Proteins / metabolism
Plant Leaves / metabolism,  radiation effects
Plant Stomata / cytology*,  enzymology,  genetics,  radiation effects*
Plant Transpiration / radiation effects
Protein Transport / radiation effects
Temperature
Vicia faba / cytology,  radiation effects
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Mutant Proteins; EC 3.6.1.-/GTP-Binding Proteins; EC 3.6.1.-/ROP2 protein, Arabidopsis
Comments/Corrections

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