Document Detail


Arabidopsis FAB1A/B is possibly involved in the recycling of auxin transporters.
MedLine Citation:
PMID:  21412048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fab1/PIKfyve produces Phosphatidylinositol 3,5-bisphosphate (PtdIns (3,5) P2) from Phosphatidylinositol 3-phosphate (PtdIns 3-P), and is involved not only in vacuole/lysosome homeostasis, but also in transporting various proteins to the vacuole or recycling proteins on the plasma membrane (PM) through the use of endosomes in a variety of eukaryotic cells. We previously demonstrated that Arabidopsis FAB1A/B functions as PtdIns 3,5-kinase in both Arabidopsis and fission yeast and plays a key role in vacuolar acidification and endocytosis. Although the conditional FAB1A/B knockdown mutant revealed an auxin-resistant phenotype to a membrane impermeable auxin, 2,4-dichlorophenoxyacetic acid (2,4-D), the mutant did not exhibit this phenotype to a membrane-permeable artificial auxin, naphthalene 1-acetic acid (NAA). The difference in the sensitivities to 2,4-D and NAA is similar to those of the auxin-resistant mutants such as aux1. Taken together, these results suggest that impairment of the function of Arabidopsis FAB1A/B might cause a defect in the membrane recycling capabilities of the auxin transporters and inhibit proper auxin transport into the cells in Arabidopsis.
Authors:
Tomoko Hirano; Masa H Sato
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Publication Detail:
Type:  Journal Article     Date:  2011-04-01
Journal Detail:
Title:  Plant signaling & behavior     Volume:  6     ISSN:  1559-2324     ISO Abbreviation:  Plant Signal Behav     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-06-15     Completed Date:  2011-10-04     Revised Date:  2012-04-27    
Medline Journal Info:
Nlm Unique ID:  101291431     Medline TA:  Plant Signal Behav     Country:  United States    
Other Details:
Languages:  eng     Pagination:  583-5     Citation Subset:  IM    
Affiliation:
Graduate School of Biostudies, Kyoto University, Kyoto, Japan. mhsato@kpu.ac.jp
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MeSH Terms
Descriptor/Qualifier:
2,4-Dichlorophenoxyacetic Acid / pharmacology
Arabidopsis / drug effects,  genetics,  metabolism*
Arabidopsis Proteins / genetics,  metabolism*
Biological Transport
Indoleacetic Acids / pharmacology
Membrane Transport Proteins / genetics,  metabolism*
Naphthaleneacetic Acids / pharmacology
Phosphotransferases (Alcohol Group Acceptor) / genetics,  metabolism*
Plants, Genetically Modified / drug effects,  genetics,  metabolism
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Indoleacetic Acids; 0/Membrane Transport Proteins; 0/Naphthaleneacetic Acids; 94-75-7/2,4-Dichlorophenoxyacetic Acid; EC 2.7.1.-/FAB1A protein, Arabidopsis; EC 2.7.1.-/FAB1B protein, Arabidopsis; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor)
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