| Arabidopsis exocyst subunits SEC8 and EXO70A1 and exocyst interactor ROH1 are involved in the localized deposition of seed coat pectin. | |
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MedLine Citation:
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PMID: 20618910 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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• Polarized deposition of cell wall pectins is a key process in Arabidopsis thaliana myxospermous seed coat development. The exocyst, an octameric secretory vesicle tethering complex, has recently been shown to be involved in the regulation of cell polarity in plants. Here, we used the Arabidopsis seed coat to study the participation of the exocyst complex in polarized pectin delivery. • We characterized the amount of pectinaceous mucilage and seed coat structure in sec8 and exo70A1 exocyst mutants. Using a yeast two-hybrid screen, we identified a new interactor of the exocyst subunit Exo70A1, termed Roh1, a member of the DUF793 protein family. • T-DNA insertions in SEC8, EXO70A1 caused considerable deviations from normal seed coat development, in particular reduced pectin deposition and defects in the formation of the central columella of seed epidermal cells. A gain-of-function mutation of ROH1 also caused reduced pectin deposition. Interestingly, we observed a systematic difference in seed coat development between primary and secondary inflorescences in wild-type plants: siliques from secondary branches produced seeds with thicker seed coats. • The participation of exocyst subunits in mucilage deposition provides direct evidence for the role of the exocyst in polarized cell wall morphogenesis. |
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Authors:
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Ivan Kulich; Rex Cole; Edita Drdová; Fatima Cvrcková; Ales Soukup; John Fowler; Viktor Zárský |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-07-02 |
Journal Detail:
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Title: The New phytologist Volume: 188 ISSN: 1469-8137 ISO Abbreviation: New Phytol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-06 Completed Date: 2011-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882884 Medline TA: New Phytol Country: England |
Other Details:
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Languages: eng Pagination: 615-25 Citation Subset: IM |
Copyright Information:
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© The Authors (2010). Journal compilation © New Phytologist Trust (2010). |
Affiliation:
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Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adhesives
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metabolism Arabidopsis / genetics, metabolism* Arabidopsis Proteins / chemistry, genetics, metabolism* DNA, Bacterial / genetics Flowers / anatomy & histology, metabolism Genes, Plant / genetics Mutagenesis, Insertional Pectins / metabolism* Phylogeny Protein Binding Protein Subunits / metabolism* Seeds / anatomy & histology, metabolism*, ultrastructure Sequence Homology, Amino Acid Vesicular Transport Proteins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Adhesives; 0/Arabidopsis Proteins; 0/BYPASS1 protein, Arabidopsis; 0/DNA, Bacterial; 0/EXO70A1 protein, Arabidopsis; 0/Pectins; 0/Protein Subunits; 0/SEC8 protein, Arabidopsis; 0/T-DNA; 0/Vesicular Transport Proteins; 9000-69-5/pectin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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