Document Detail

Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis.
MedLine Citation:
PMID:  9952446     Owner:  NLM     Status:  MEDLINE    
The Arabidopsis GA3 cDNA was expressed in yeast (Saccharomyces cerevisiae) and the ability of the transformed yeast cells to metabolize ent-kaurene was tested. We show by full-scan gas chromatography-mass spectrometry that the transformed cells produce ent-kaurenoic acid, and demonstrate that the single enzyme GA3 (ent-kaurene oxidase) catalyzes the three steps of gibberellin biosynthesis from ent-kaurene to ent-kaurenoic acid.
C A Helliwell; A Poole; W J Peacock; E S Dennis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  119     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-04-15     Completed Date:  1999-04-15     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  507-10     Citation Subset:  IM    
Commonwealth Scientific and Industrial Research Organization, Plant Industry, G.P.O. Box 1600, Canberra, ACT 2601, Australia.
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MeSH Terms
Arabidopsis / enzymology*,  genetics,  metabolism
Cytochrome P-450 Enzyme System / genetics,  metabolism*
DNA, Complementary / genetics
DNA, Plant / genetics
Diterpenes / metabolism
Diterpenes, Kaurane*
Gene Expression
Genes, Plant
Gibberellins / biosynthesis*,  genetics
Oxygenases / genetics,  metabolism*
Saccharomyces cerevisiae / genetics
Transformation, Genetic
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Plant; 0/Diterpenes; 0/Diterpenes, Kaurane; 0/Gibberellins; 34424-57-2/kaurene; 6730-83-2/kaurenoic acid; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.13.-/Oxygenases; EC 1.14.12.-/ent-kaurene oxidase

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