Document Detail

Arabidopsis cytosolic glutamine synthetase AtGLN1;1 is a potential substrate of AtCRK3 involved in leaf senescence.
MedLine Citation:
PMID:  16472779     Owner:  NLM     Status:  MEDLINE    
While considerable progress has been achieved in plant CDPK studies in the past decade, there is relatively no information about the potential substrates of CRKs. In this report, a yeast two-hybrid screen was performed with truncated form of AtCRK3 as bait to identify its interacting proteins in an effort to dissect its physiological roles. One gene encoding cytosolic glutamine synthetase AtGLN1;1 was isolated. Further analyses indicated that AtGLN1;1 could interact specifically with AtCRK3 and the kinase domain of AtCRK3 and the catalytic domain of AtGLN1;1 were responsible for such interaction, respectively. Furthermore, in vitro and in vivo co-immunoprecipitation results strongly supported that they could physically interact with each other. Phosphorylation assays revealed that AtGLN1;1 could be specifically phosphorylated by AtCRK3 in vitro. All the results demonstrate that AtGLN1;1 may be a substrate of AtCRK3. In addition, both AtGLN1;1 and AtCRK3 could be induced by natural or artificially induced leaf senescence, implying that such interaction may be involved in the regulation of nitrogen remobilization during leaf senescence.
Rong-Jun Li; Wei Hua; Ying-Tang Lu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-02
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  342     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-20     Completed Date:  2006-04-13     Revised Date:  2012-08-01    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  119-26     Citation Subset:  IM    
Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
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MeSH Terms
Arabidopsis / enzymology*,  genetics,  growth & development
Arabidopsis Proteins / genetics,  metabolism*
CDC2 Protein Kinase / genetics,  metabolism*
Cytosol / enzymology*
Gene Expression Regulation, Plant / genetics
Glutamate-Ammonia Ligase / genetics,  metabolism*
Plant Leaves / enzymology,  genetics,  growth & development*
Protein Binding
Protozoan Proteins / genetics,  metabolism*
Saccharomyces cerevisiae / genetics,  metabolism
Substrate Specificity
Two-Hybrid System Techniques
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Protozoan Proteins; EC Protein Kinase; EC Ligase

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