| Arabidopsis MICROTUBULE-ASSOCIATED PROTEIN18 functions in directional cell growth by destabilizing cortical microtubules. | |
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MedLine Citation:
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PMID: 17337629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microtubule-associated proteins (MAPs) play important roles in the regulation of microtubule function in cells. We describe Arabidopsis thaliana MAP18, which binds to microtubules and inhibits tubulin polymerization in vitro and colocalizes along cortical microtubules as patches of dot-like structures. MAP18 is expressed mostly in the expanding cells. Cells overexpressing MAP18 in Arabidopsis exhibit various growth phenotypes with loss of polarity. Cortical microtubule arrays were significantly altered in cells either overexpressing MAP18 or where it had been downregulated by RNA interference (RNAi). The cortical microtubules were more sensitive to treatment with microtubule-disrupting drugs when MAP18 was overexpressed, but more resistant when MAP18 was eliminated in cells expressing MAP18 RNAi. Our study demonstrated that MAP18 may play a role in regulating directional cell growth and cortical microtubule organization by destabilizing microtubules. |
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Authors:
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Xia Wang; Lei Zhu; Baoquan Liu; Che Wang; Lifeng Jin; Qian Zhao; Ming Yuan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-03-02 |
Journal Detail:
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Title: The Plant cell Volume: 19 ISSN: 1040-4651 ISO Abbreviation: Plant Cell Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-05-01 Completed Date: 2007-07-06 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9208688 Medline TA: Plant Cell Country: United States |
Other Details:
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Languages: eng Pagination: 877-89 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100094, China. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AAM53961; AAR29265; AAS17876; BAC41928; CAA65228 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Arabidopsis / cytology*, drug effects, genetics Arabidopsis Proteins / chemistry, genetics, isolation & purification, metabolism* Cell Polarity* / drug effects Cell Size / drug effects Dinitrobenzenes / pharmacology Gene Expression Profiling Gene Expression Regulation, Plant / drug effects Genes, Plant Microtubule-Associated Proteins / chemistry, genetics, isolation & purification, metabolism* Microtubules / drug effects, metabolism* Molecular Sequence Data Plant Roots / cytology, growth & development Plants, Genetically Modified Protein Binding / drug effects Protein Transport / drug effects RNA Interference Recombinant Proteins / isolation & purification, metabolism Sulfanilamides / pharmacology Tubulin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/Dinitrobenzenes; 0/MAP18 protein, Arabidopsis; 0/Microtubule-Associated Proteins; 0/Recombinant Proteins; 0/Sulfanilamides; 0/Tubulin; 19044-88-3/oryzalin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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