Document Detail


Arabidopsis MICROTUBULE-ASSOCIATED PROTEIN18 functions in directional cell growth by destabilizing cortical microtubules.
MedLine Citation:
PMID:  17337629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microtubule-associated proteins (MAPs) play important roles in the regulation of microtubule function in cells. We describe Arabidopsis thaliana MAP18, which binds to microtubules and inhibits tubulin polymerization in vitro and colocalizes along cortical microtubules as patches of dot-like structures. MAP18 is expressed mostly in the expanding cells. Cells overexpressing MAP18 in Arabidopsis exhibit various growth phenotypes with loss of polarity. Cortical microtubule arrays were significantly altered in cells either overexpressing MAP18 or where it had been downregulated by RNA interference (RNAi). The cortical microtubules were more sensitive to treatment with microtubule-disrupting drugs when MAP18 was overexpressed, but more resistant when MAP18 was eliminated in cells expressing MAP18 RNAi. Our study demonstrated that MAP18 may play a role in regulating directional cell growth and cortical microtubule organization by destabilizing microtubules.
Authors:
Xia Wang; Lei Zhu; Baoquan Liu; Che Wang; Lifeng Jin; Qian Zhao; Ming Yuan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-02
Journal Detail:
Title:  The Plant cell     Volume:  19     ISSN:  1040-4651     ISO Abbreviation:  Plant Cell     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-05-01     Completed Date:  2007-07-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  877-89     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AAM53961;  AAR29265;  AAS17876;  BAC41928;  CAA65228
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Arabidopsis / cytology*,  drug effects,  genetics
Arabidopsis Proteins / chemistry,  genetics,  isolation & purification,  metabolism*
Cell Polarity* / drug effects
Cell Size / drug effects
Dinitrobenzenes / pharmacology
Gene Expression Profiling
Gene Expression Regulation, Plant / drug effects
Genes, Plant
Microtubule-Associated Proteins / chemistry,  genetics,  isolation & purification,  metabolism*
Microtubules / drug effects,  metabolism*
Molecular Sequence Data
Plant Roots / cytology,  growth & development
Plants, Genetically Modified
Protein Binding / drug effects
Protein Transport / drug effects
RNA Interference
Recombinant Proteins / isolation & purification,  metabolism
Sulfanilamides / pharmacology
Tubulin / metabolism
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Dinitrobenzenes; 0/MAP18 protein, Arabidopsis; 0/Microtubule-Associated Proteins; 0/Recombinant Proteins; 0/Sulfanilamides; 0/Tubulin; 19044-88-3/oryzalin
Comments/Corrections

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