| Arabidopsis IAP-like protein lacks a BIR domain and attenuates cell death in plant and animal systems. | |
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MedLine Citation:
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PMID: 21926169 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A novel Arabidopsis thaliana inhibitor of apoptosis was identified by sequence homology to other known inhibitor of apoptosis (IAP) proteins. Arabidopsis IAP-like protein (AtILP) contained a carboxy-terminal RING finger domain but lacked a baculovirus IAP repeat (BIR) domain, which is essential for anti-apoptotic activity in other IAP family members. The expression of AtILP in HeLa cells conferred resistance against tumor necrosis factor (TNF)-α/ActD-induced apoptosis through the inactivation of caspase activity. In contrast to the carboxy-terminal RING domain of AtILP, which did not inhibit the activity of caspase-3, the N-terminal region, despite displaying no homology to known BIR domains, potently inhibited the activity of caspase-3 in vitro and blocked TNF-α/ActD-induced apoptosis. The anti-apoptotic activity of the AtILP N-terminal domain observed in plants was reproduced in an animal system. Transgenic Arabidopsis lines over-expressing AtILP exhibited anti-apoptotic activity when challenged with the fungal toxin fumonisin B1, an agent that induces apoptosis-like cell death in plants. In AtIPL transgenic plants, suppression of cell death was accompanied by inhibition of caspase activation and DNA fragmentation. Over-expression of AtILP also attenuated effector protein-induced cell death and increased the growth of an avirulent bacterial pathogen. The current results demonstrated the existence of a novel plant IAP-like protein that prevents caspase activation in Arabidopsis, and showed that a plant anti-apoptosis gene functions similarly in plant and animal systems. |
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Authors:
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Woe Yeon Kim; Sun Yong Lee; Young Jun Jung; Ho Byoung Chae; Ganesh M Nawkar; Mi Rim Shin; Sun Young Kim; Jin Ho Park; Chang Ho Kang; Yong Hoon Chi; Il Pyung Ahn; Dae Jin Yun; Kyun Oh Lee; Young-Myeong Kim; Min Gab Kim; Sang Yeol Lee |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-16 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: - ISSN: 1083-351X ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Gyeongsang National University, Korea, Republic of; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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