Document Detail


The Arabidopsis glucosyltransferase UGT76B1 conjugates isoleucic acid and modulates plant defense and senescence.
MedLine Citation:
PMID:  22080599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plants coordinate and tightly regulate pathogen defense by the mostly antagonistic salicylate (SA)- and jasmonate (JA)-mediated signaling pathways. Here, we show that the previously uncharacterized glucosyltransferase UGT76B1 is a novel player in this SA-JA signaling crosstalk. UGT76B1 was selected as the top stress-induced isoform among all 122 members of the Arabidopsis thaliana UGT family. Loss of UGT76B1 function leads to enhanced resistance to the biotrophic pathogen Pseudomonas syringae and accelerated senescence but increased susceptibility toward necrotrophic Alternaria brassicicola. This is accompanied by constitutively elevated SA levels and SA-related marker gene expression, whereas JA-dependent markers are repressed. Conversely, UGT76B1 overexpression has the opposite effect. Thus, UGT76B1 attenuates SA-dependent plant defense in the absence of infection, promotes the JA response, and delays senescence. The ugt76b1 phenotypes were SA dependent, whereas UGT76B1 overexpression indicated that this gene possibly also has a direct effect on the JA pathway. Nontargeted metabolomic analysis of UGT76B1 knockout and overexpression lines using ultra-high-resolution mass spectrometry and activity assays with the recombinant enzyme led to the ab initio identification of isoleucic acid (2-hydroxy-3-methyl-pentanoic acid) as a substrate of UGT76B1. Exogenously applied isoleucic acid increased resistance against P. syringae infection. These findings indicate a novel link between amino acid-related molecules and plant defense that is mediated by small-molecule glucosylation.
Authors:
Veronica von Saint Paul; Wei Zhang; Basem Kanawati; Birgit Geist; Theresa Faus-Kessler; Philippe Schmitt-Kopplin; Anton R Schäffner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-11-11
Journal Detail:
Title:  The Plant cell     Volume:  23     ISSN:  1532-298X     ISO Abbreviation:  Plant Cell     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-12-28     Completed Date:  2012-08-21     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4124-45     Citation Subset:  IM    
Affiliation:
Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, 85764 Neuherberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Aging / genetics
Alternaria / pathogenicity
Arabidopsis / microbiology,  physiology*
Arabidopsis Proteins / genetics,  metabolism*
Base Sequence
Cyclopentanes / metabolism
Gene Expression Regulation, Plant
Gene Knockout Techniques
Genetic Markers
Glucosyltransferases / genetics,  metabolism*
Glycosyltransferases / genetics,  metabolism*
Host-Pathogen Interactions
Molecular Sequence Data
Oxylipins / metabolism
Pentanoic Acids / metabolism,  pharmacology
Plant Diseases / genetics
Plant Roots / drug effects
Pseudomonas syringae / pathogenicity
Salicylic Acid / metabolism
Signal Transduction
Substrate Specificity
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Cyclopentanes; 0/Genetic Markers; 0/Oxylipins; 0/Pentanoic Acids; 488-15-3/2-hydroxy-3-methylvaleric acid; 69-72-7/Salicylic Acid; 6RI5N05OWW/jasmonic acid; EC 2.4.-/Glycosyltransferases; EC 2.4.-/UGT76B1 protein, Arabidopsis; EC 2.4.1.-/Glucosyltransferases
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