Document Detail


Arabidopsis DUO POLLEN3 is a key regulator of male germline development and embryogenesis.
MedLine Citation:
PMID:  19638475     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Male germline development in angiosperms produces the pair of sperm cells required for double fertilization. A key regulator of this process in Arabidopsis thaliana is the male germline-specific transcription factor DUO POLLEN1 (DUO1) that coordinates germ cell division and gamete specification. Here, we uncover the role of DUO3, a nuclear protein that has a distinct, but overlapping role with DUO1 in male germline development. DUO3 is a conserved protein in land plants and is related to GON-4, a cell lineage regulator of gonadogenesis in Caenorhabditis elegans. Mutant duo3-1 germ cells either fail to divide or show a delay in division, and we show that, unlike DUO1, DUO3 promotes entry into mitosis independent of the G2/M regulator CYCB1;1. We also show that DUO3 is required for the expression of a subset of germline genes under DUO1 control and that like DUO1, DUO3 is essential for sperm cell specification and fertilization. Furthermore, we demonstrate an essential sporophytic role for DUO3 in cell division and embryo patterning. Our findings demonstrate essential developmental roles for DUO3 in cell cycle progression and cell specification in both gametophytic and sporophytic tissues.
Authors:
Lynette Brownfield; Said Hafidh; Anjusha Durbarry; Hoda Khatab; Anna Sidorova; Peter Doerner; David Twell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-28
Journal Detail:
Title:  The Plant cell     Volume:  21     ISSN:  1040-4651     ISO Abbreviation:  Plant Cell     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-08-27     Completed Date:  2009-10-27     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1940-56     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Leicester, Leicester LE1 7RH, United Kingdom.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/FJ461625;  FJ461626;  FJ461627; RefSeq/NP_191605
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / cytology*,  embryology*,  genetics
Arabidopsis Proteins / genetics,  physiology*
Cell Cycle / genetics,  physiology
Cell Division / genetics,  physiology
Computational Biology
Embryonic Development / genetics,  physiology*
Gene Expression Regulation, Plant / genetics,  physiology
Microscopy, Confocal
Molecular Sequence Data
Pollen / cytology,  genetics
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
//Biotechnology and Biological Sciences Research Council; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins
Comments/Corrections
Comment In:
Plant Signal Behav. 2009 Dec;4(12):1159-62   [PMID:  20514235 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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