Document Detail


Arabidopsis ACCELERATED CELL DEATH2 modulates programmed cell death.
MedLine Citation:
PMID:  16387834     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Arabidopsis thaliana chloroplast protein ACCELERATED CELL DEATH2 (ACD2) modulates the amount of programmed cell death (PCD) triggered by Pseudomonas syringae and protoporphyrin IX (PPIX) treatment. In vitro, ACD2 can reduce red chlorophyll catabolite, a chlorophyll derivative. We find that ACD2 shields root protoplasts that lack chlorophyll from light- and PPIX-induced PCD. Thus, chlorophyll catabolism is not obligatory for ACD2 anti-PCD function. Upon P. syringae infection, ACD2 levels and localization change in cells undergoing PCD and in their close neighbors. Thus, ACD2 shifts from being largely in chloroplasts to partitioning to chloroplasts, mitochondria, and, to a small extent, cytosol. ACD2 protects cells from PCD that requires the early mitochondrial oxidative burst. Later, the chloroplasts of dying cells generate NO, which only slightly affects cell viability. Finally, the mitochondria in dying cells have dramatically altered movements and cellular distribution. Overproduction of both ACD2 (localized to mitochondria and chloroplasts) and ascorbate peroxidase (localized to chloroplasts) greatly reduces P. syringae-induced PCD, suggesting a pro-PCD role for mitochondrial and chloroplast events. During infection, ACD2 may bind to and/or reduce PCD-inducing porphyrin-related molecules in mitochondria and possibly chloroplasts that generate reactive oxygen species, cause altered organelle behavior, and activate a cascade of PCD-inducing events.
Authors:
Nan Yao; Jean T Greenberg
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2005-12-30
Journal Detail:
Title:  The Plant cell     Volume:  18     ISSN:  1040-4651     ISO Abbreviation:  Plant Cell     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-02     Completed Date:  2006-08-04     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  397-411     Citation Subset:  IM    
Affiliation:
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/X98926
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MeSH Terms
Descriptor/Qualifier:
Apoptosis* / drug effects,  radiation effects
Apoptosis Regulatory Proteins / metabolism*
Arabidopsis / cytology*,  drug effects,  metabolism*,  microbiology
Arabidopsis Proteins / metabolism*
Bacterial Infections / metabolism
Chlorophyll / metabolism
Chloroplasts / metabolism
Gene Expression
Herbicides / pharmacology
Hydrogen Peroxide / metabolism
Light
Mitochondria / metabolism
Molecular Sequence Data
Nitric Oxide / metabolism
Oxidoreductases / metabolism*
Phenotype
Plant Diseases / microbiology
Plant Leaves / drug effects,  microbiology,  ultrastructure
Plant Roots / ultrastructure
Protein Transport
Protoporphyrins / pharmacology
Pseudomonas syringae / growth & development,  pathogenicity
Virulence
Grant Support
ID/Acronym/Agency:
5R01 GM54292/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/Arabidopsis Proteins; 0/Herbicides; 0/Protoporphyrins; 10102-43-9/Nitric Oxide; 1406-65-1/Chlorophyll; 553-12-8/protoporphyrin IX; 7722-84-1/Hydrogen Peroxide; EC 1.-/Oxidoreductases; EC 1.3.7.-/ACD2 protein, Arabidopsis
Comments/Corrections
Erratum In:
Plant Cell. 2007 Aug;19(8):2690

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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