| Aqueous extraction of beta-glucuronidase from transgenic canola: kinetics and microstructure. | |
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MedLine Citation:
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PMID: 12467466 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aqueous extraction kinetics of recombinant beta-glucuronidase (rGUS) from transgenic canola (Brassica napus) was investigated in terms of the particle size and microstructural characteristics resulting from canola seed processing. The canola had been transformed to express recombinant GUS intracellulary in the seed, and electron microscopy showed that the cells are distributed among (1) disrupted cells in a thin layer at or adjacent to the particle surface, (2) disrupted cells within the interior, and (3) intact cells within the interior. A simple compartmental model containing two extractable pools and a third nonextractable pool fitted the batch extraction results very well. Comparing the rate constants from the model to estimates of expected transport rates from the observed cell fractions showed that the two extractable pools roughly correspond to the two disrupted cell fractions. Both flaking, causing more extensive cell wall damage throughout the seed, and grinding, increasing the total surface area, increase the size of the first pool and, therefore, the extraction yield. Mass transfer from the same type of pool from two types of processed seed behaved similarly. GUS extraction from the first extractable pool is 10-20 times faster (<1 min) than from the second extractable pool. |
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Authors:
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Yun Bai; Zivko L Nikolov; Charles E Glatz |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biotechnology progress Volume: 18 ISSN: 8756-7938 ISO Abbreviation: Biotechnol. Prog. Publication Date: 2002 Nov-Dec |
Date Detail:
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Created Date: 2002-12-06 Completed Date: 2003-07-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: United States |
Other Details:
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Languages: eng Pagination: 1301-5 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, Iowa State University, Ames, Iowa, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Brassica napus
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enzymology*,
metabolism Glucuronidase / biosynthesis, isolation & purification* Methods Microscopy, Electron Plants, Genetically Modified / enzymology*, metabolism Recombinant Proteins / biosynthesis, isolation & purification Seeds / cytology, enzymology, metabolism Water |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins; 7732-18-5/Water; EC 3.2.1.31/Glucuronidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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