Document Detail


Aqueous extract of ginger shows antiproliferative activity through disruption of microtubule network of cancer cells.
MedLine Citation:
PMID:  20647029     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ginger has a long history of use as traditional medicine for varied human disease. Our present study has shown that the aqueous extract of ginger (GAE) interacts directly with cellular microtubules and disrupts its structure and induces apoptosis of cancer cells as well. The IC(50) values of GAE, as determined from cell viability experiment on human non-small lung epithelium cancer (A549) cells and human cervical epithelial carcinoma (HeLa), were 239.4+7.4 and 253.4+8.9 μg/ml, respectively. It has been found that the apoptosis of A549 cells by GAE is mediated by up regulation of tumor suppressor gene p53 and alteration of the normal Bax/Bcl-2 ratio followed by down regulation of cellular pro-caspase3. The morphological change of cells upon GAE treatment has also been demonstrated. Both the structural and functional properties of tubulin and microtubule were lost, as confirmed by both ex vivo and invitro experiments. The major component of GAE is poly-phenols (around 2.5%), which consist of ∼ 80% flavones and flavonols. Poly-phenolic compounds are well known to have anti-mitotic properties, and may be further screened for the development of a potential anti-cancer agent.
Authors:
Diptiman Choudhury; Amlan Das; Abhijit Bhattacharya; Gopal Chakrabarti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-18
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  48     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-10     Completed Date:  2010-12-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2872-80     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Biotechnology and Dr BC Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, WB 700 019, India.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis / drug effects
Blotting, Western
Cell Death / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Survival / drug effects
DNA, Neoplasm / biosynthesis
Flavones / chemistry,  pharmacology
Flavonols / chemistry,  pharmacology
Flow Cytometry
Ginger / chemistry*
Hela Cells
Humans
Membrane Potentials / drug effects
Microtubules / drug effects*,  pathology,  ultrastructure
Mitochondria / drug effects
Neoplasms / drug therapy*,  pathology*
Phenols / chemistry,  pharmacology
Plant Extracts / pharmacology
Tubulin / drug effects,  metabolism
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/DNA, Neoplasm; 0/Flavones; 0/Flavonols; 0/Phenols; 0/Plant Extracts; 0/Tubulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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