| Aqueous extract of Phyllanthus niruri (Euphorbiaceae) enhances the phenotypic and functional maturation of bone marrow-derived dendritic cells and their antigen-presentation function. | |
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MedLine Citation:
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PMID: 20095802 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Decoctions of Phyllanthus niruri (PN) (Fam. Euphorbiaceae) is promoted in traditional medicine of Africa, Asia, and South America as beneficial supplement for different infectious diseases, especially for viral hepatitis, tumor, and for immune compromised patients. This stimulated the interest in understanding the mechanisms by which the whole extract of the plant could stimulate the immune system. Dendritic cells (DCs) are professional antigen-presenting cells and provide a link between the innate and the adaptive immune responses. In the present study, the effects of lyophilized aqueous extract of PN on structural and functional maturation of murine bone marrow-derived DCs (BM-DCs) were investigated. Bone marrow cells were cultured in the presence of granulocyte macrophage-colony stimulating factor and interleukin-4 (IL-4) and the generated immature DCs were stimulated with PN (25, 50, and 100 microg/mL) or lipopolysaccharide (10 microg/mL) for 48 h. Results showed that treatment with PN increased the expression of major histocompatibility complex-II and the various makers for DCs maturation (CD40), activation (CD83), and costimulation (CD86) in a concentration-dependent manner. Consistent with the increase in phenotypic makers, functional maturation assay showed that treatment of BM-DCs with PN caused a decrease in fluorescein isothiocyanate-dextran pinocytosis and an increase in IL-12 in the supernatant. In a transgenic T-cell activation model, PN-treated BM-DCs presented Ova antigen to Ova-specific CD8(+) T cells from OT-1 mice more efficiently as demonstrated by increased T-cells proliferation and IL-2 production. Therefore, PN enhances the structural and functional maturation of BM-DCs and their antigen-presenting function. These effects are relevant in immunodeficient conditions, tumor control, and in infectious diseases. |
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Authors:
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Chukwuemeka S Nworu; Peter A Akah; Festus B C Okoye; Charles O Esimone |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Immunopharmacology and immunotoxicology Volume: 32 ISSN: 1532-2513 ISO Abbreviation: Immunopharmacol Immunotoxicol Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-09 Completed Date: 2010-12-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8800150 Medline TA: Immunopharmacol Immunotoxicol Country: England |
Other Details:
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Languages: eng Pagination: 393-401 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Nigeria. csnworu@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigen Presentation / drug effects*, immunology Antigens, CD / analysis, immunology Antigens, CD40 / analysis, immunology Antigens, CD86 / analysis, immunology CD8-Positive T-Lymphocytes / drug effects, immunology Cell Differentiation / drug effects*, immunology Dendritic Cells / drug effects*, immunology Granulocyte-Macrophage Colony-Stimulating Factor / immunology Immunoglobulins / analysis, immunology Interleukin-12 / analysis, immunology Interleukin-4 / immunology Lipopolysaccharides / immunology Major Histocompatibility Complex / drug effects, immunology Membrane Glycoproteins / analysis, immunology Mice Mice, Inbred C57BL Mice, Transgenic Phyllanthus* Pinocytosis / drug effects, immunology Plant Extracts / immunology, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, CD40; 0/Antigens, CD86; 0/CD83 antigen; 0/Immunoglobulins; 0/Lipopolysaccharides; 0/Membrane Glycoproteins; 0/Plant Extracts; 187348-17-0/Interleukin-12; 207137-56-2/Interleukin-4; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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