Document Detail

Aquaporin expression in the human intervertebral disc.
MedLine Citation:
PMID:  18247144     Owner:  NLM     Status:  MEDLINE    
The nucleus pulposus (NP) of the human intervertebral disc (IVD) is a hyperosmotic tissue that is subjected to daily dynamic compressive loads. In order to survive within this environment the resident chondrocyte-like cells must be able to control their cell volume, whilst also controlling the anabolism and catabolism of their extra-cellular matrix. Recent studies have demonstrated expression of a range of bi-directional, transmembrane water and solute transporters, named aquaporins (AQPs), within chondrocytes of articular cartilage. The aim of this study was to use immunohistochemsitry to investigate the expression of aquaporins 1, 2 and 3 within the human IVD. Results demonstrated expression of both AQP-1 and -3 by cells within the NP and inner annulus fibrosus (AF), while outer AF cells lacked expression of AQP-1 and showed very low numbers of AQP-3 immunopositive cells. Cells from all regions were negative for AQP-2. Therefore this study demonstrates similarities in the phenotype of NP cells and articular chondrocytes, which may be due to similarities in tissue osmolarity and mechanobiology. The decrease in expression of AQPs from the NP to the outer AF may signify changes in cellular phenotype in response to differences in mechanbiology, osmolarity and hydration between the gelatinous NP and the fibrous AF.
S M Richardson; R Knowles; D Marples; J A Hoyland; A Mobasheri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-06
Journal Detail:
Title:  Journal of molecular histology     Volume:  39     ISSN:  1567-2379     ISO Abbreviation:  J. Mol. Histol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-04-22     Completed Date:  2008-10-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101193653     Medline TA:  J Mol Histol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  303-9     Citation Subset:  IM    
Tissue Injury and Repair Group, School of Clinical and Laboratory Sciences, Faculty of Medical and Human Sciences, The University of Manchester, Manchester M13 9PT, UK.
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MeSH Terms
Aquaporin 1 / metabolism
Aquaporin 2 / metabolism
Aquaporin 3 / metabolism
Aquaporins / metabolism*
Intervertebral Disk / anatomy & histology,  cytology,  metabolism*
Middle Aged
Grant Support
//Biotechnology and Biological Sciences Research Council; //Medical Research Council
Reg. No./Substance:
0/Aquaporin 2; 0/Aquaporins; 146410-94-8/Aquaporin 1; 158801-98-0/Aquaporin 3

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