Document Detail

Aquaporin expression and cell volume regulation in the SV40 immortalized rat submandibular acinar cell line.
MedLine Citation:
PMID:  17021794     Owner:  NLM     Status:  MEDLINE    
The amount of aquaporins present and the cellular ability to perform regulatory volume changes are likely to be important for fluid secretions from exocrine glands. In this work these phenomena were studied in an SV40 immortalized rat submandibular acinar cell line. The regulatory cell volume characteristics have not previously been determined in these cells. Cell volume regulation following hyposmotic exposure and aquaporin induction was examined with Coulter counter methodology, radioactive efflux studies, fura-2 fluorescence, and polymerase chain reaction and Western blot techniques. Cell volume regulation was inhibited by the K(+) channel antagonists quinine and BaCl(2) and the Cl(-) channel blocker 5-nitro-2-(3-phenypropylamino)benzoic acid. A concomitant increase in cellular (3)H-taurine release and Ca(2+) concentration was also observed. Chelation of both intra- and extracellular Ca(2+) with EGTA and the Ca(2+) ionophore A23187 did not, however, affect cell volume regulation. Aquaporin 5 (AQP5) mRNA and protein levels were upregulated in hyperosmotic conditions and downregulated upon return to isosmotic solutions, but were reduced by the mitogen-activated ERK-activating kinase (MEK) inhibitor U0126. A 24-h MEK inhibition also diminished hyposmotically induced cell swelling and cell volume regulation. In conclusion, it was determined that regulatory volume changes in this immortalized cell line are due to KCl and taurine efflux. In conditions that increased AQP5 levels, the cells showed a faster cell swelling and a more complete volume recovery following hyposmotic exposure. This response could be overturned by MEK inhibition.
Ann-Kristin Hansen; Hilde Kanli Galtung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-05
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  453     ISSN:  0031-6768     ISO Abbreviation:  Pflugers Arch.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-19     Completed Date:  2007-07-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  787-96     Citation Subset:  IM    
Institute of Oral Biology, University of Oslo, Oslo, Norway.
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MeSH Terms
Antigens, Polyomavirus Transforming / genetics*
Aquaporin 5 / genetics*,  metabolism*
Calcimycin / pharmacology
Calcium / metabolism
Cell Line, Transformed
Cell Size
Chelating Agents / pharmacology
Egtazic Acid / pharmacology
Ionophores / pharmacology
MAP Kinase Signaling System / physiology
Potassium Chloride / pharmacokinetics
Submandibular Gland / cytology*
Taurine / pharmacokinetics
Tritium / diagnostic use
Water-Electrolyte Balance / drug effects,  physiology*
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/Aqp5 protein, rat; 0/Aquaporin 5; 0/Chelating Agents; 0/Ionophores; 10028-17-8/Tritium; 107-35-7/Taurine; 52665-69-7/Calcimycin; 67-42-5/Egtazic Acid; 7440-70-2/Calcium; 7447-40-7/Potassium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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