Document Detail

Aquaporin-4 water channel expression by thymoma of patients with and without myasthenia gravis.
MedLine Citation:
PMID:  20728226     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Neuromyelitis optica (NMO) is a serious idiopathic inflammatory demyelinating disorder characterized by acute transverse myelitis and optic neuritis. A significant proportion of NMO patients are seropositive for NMO-IgG, an autoantibody targeting aquaporin-4 (AQP4) water channel. Paraneoplastic NMO associated various tumors were recently reported. AIM: We studied the expression of AQP4 by thymoma from patients with and without myasthenia gravis (MG). METHODS: Thymoma obtained from thymomectomy in patients with and without MG were studied by immunohistochemistry and western blot. RESULTS: Ten thymoma patients (9 with MG) and two control patients without thymoma or MG were studied. Immunohistochemistry revealed AQP4 immunoreactivity in cell membrane of thymoma cells from all ten thymoma specimens whereas thymic tissues from patients without thymoma or MG were negative for AQP4 immunoreactivity. Western blot revealed that lysates of nine of the ten thymoma specimens reacted with anti-human AQP4 antibody with a band of ~30 kDa compatible with the molecular weight of AQP4. Interestingly, immunofluorescence revealed that IgG isolated from 2 NMO patients seropositive for NMO-IgG bound to cell membrane of thymoma cells from all ten thymoma specimens while IgG from healthy control subject did not. CONCLUSION: Thymoma cells of patients with and without MG express AQP4. AQP4 autoantibodies from serum of NMO patients bound to AQP4 expressed on thymoma cell membrane.
K H Chan; J S C Kwan; P W L Ho; S L Ho; W H Chui; A C Y Chu; J W M Ho; W Y Zhang; M H W Kung
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-21
Journal Detail:
Title:  Journal of neuroimmunology     Volume:  227     ISSN:  1872-8421     ISO Abbreviation:  J. Neuroimmunol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-20     Completed Date:  2010-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8109498     Medline TA:  J Neuroimmunol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  178-84     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
University Department of Medicine, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong.
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MeSH Terms
Aquaporin 4 / biosynthesis*,  genetics
Gene Expression Regulation / immunology
Middle Aged
Myasthenia Gravis / immunology,  metabolism*,  pathology
Thymoma / immunology,  metabolism*,  pathology
Thymus Neoplasms / immunology,  metabolism*,  pathology
Reg. No./Substance:
0/AQP4 protein, human; 0/Aquaporin 4

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