Document Detail


Aquaporin-3 expression in human fetal airway epithelial progenitor cells.
MedLine Citation:
PMID:  16043462     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Airway epithelium stem cells have not yet been prospectively identified, but it is generally assumed that both secretory and basal cells have the capacity to divide and differentiate. Previously, we developed a test for progenitor cells of the human airway epithelium, relying on the transplantation of fetal respiratory tissues into immunodeficient mice. In this study, we hypothesized that airway-repopulating epithelial progenitors can be marked with surface antigens, and we screened an array of such candidate markers, including lectin ligands, the CD44 and CD166 adhesion molecules, and the aquaporin-3 (AQP3) water channel. We observed that AQP3 is selectively expressed on the surface of basal cells, allowing the separation by flow cytometry of AQP3+ basal cells and AQP3- ciliated and secretory cells. Functional evaluation of sorted cells in vivo showed that AQP3+ cells can restore a normal pseudostratified, mucociliary epithelium as well as submucosal glands. AQP3- cells are also endowed with a similar potential, although faster engraftment suggests their inclusion of more committed progenitors. These results show that stem cell candidates in the human tracheo-bronchial mucosa can be positively selected with a novel marker but also, for the first time, that epithelial progenitors exist among both basal and suprabasal cell subsets within the human airway.
Authors:
Aurélie Avril-Delplanque; Ibrahim Casal; Nicolas Castillon; Jocelyne Hinnrasky; Edith Puchelle; Bruno Péault
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  23     ISSN:  1066-5099     ISO Abbreviation:  Stem Cells     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-26     Completed Date:  2005-11-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  992-1001     Citation Subset:  IM    
Affiliation:
INSERM u506, Hôpital Paul Brousse, Villejuif, France.
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MeSH Terms
Descriptor/Qualifier:
Activated-Leukocyte Cell Adhesion Molecule / biosynthesis
Animals
Antigens, CD44 / biosynthesis
Aquaporin 3
Aquaporins / biosynthesis*
Cell Differentiation
Cell Separation
Cell Transplantation
Epithelial Cells / cytology*
Flow Cytometry
Gene Expression Regulation, Developmental*
Humans
Immunohistochemistry
Lectins / metabolism
Ligands
Lung / embryology*
Mice
Mice, SCID
Stem Cells / cytology*
Trachea / embryology*
Chemical
Reg. No./Substance:
0/AQP3 protein, human; 0/Activated-Leukocyte Cell Adhesion Molecule; 0/Antigens, CD44; 0/Aqp3 protein, mouse; 0/Aquaporins; 0/Lectins; 0/Ligands; 158801-98-0/Aquaporin 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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