Document Detail


Aquaporin 3 cloned from Xenopus laevis is regulated by the cystic fibrosis transmembrane conductance regulator.
MedLine Citation:
PMID:  10869574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cystic fibrosis transmembrane conductance regulator (CFTR) is essential for epithelial electrolyte transport and has been shown to be a regulator of epithelial Na(+), K(+), and Cl(-) channels. CFTR also enhances osmotic water permeability when activated by cAMP. This was detected initially in Xenopus oocytes and is also present in human airway epithelial cells, however, the mechanisms remain obscure. Here, we show that CFTR activates aquaporin 3 expressed endogenously and exogenously in oocytes of Xenopus laevis. The interaction requires stimulation of wild type CFTR by cAMP and an intact first nucleotide binding domain as demonstrated for other CFTR-protein interactions.
Authors:
R Schreiber; H Pavenstädt; R Greger; K Kunzelmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  475     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-07-24     Completed Date:  2000-07-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  291-5     Citation Subset:  IM    
Affiliation:
Department of Physiology and Pharmacology, University of Queensland, St. Lucia, Brisbane, Australia.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Aquaporin 3
Aquaporins / genetics*,  metabolism*
Cloning, Molecular
Cyclic AMP / metabolism
Cystic Fibrosis Transmembrane Conductance Regulator / metabolism*
Female
Humans
Molecular Sequence Data
Oocytes
Xenopus laevis
Chemical
Reg. No./Substance:
0/Aquaporins; 0/CFTR protein, human; 126880-72-6/Cystic Fibrosis Transmembrane Conductance Regulator; 158801-98-0/Aquaporin 3; 60-92-4/Cyclic AMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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