Document Detail


Aptamer-targeted magnetic nanospheres as a solid-phase extraction sorbent for determination of ochratoxin A in food samples.
MedLine Citation:
PMID:  21890142     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A sorbent based on the aptamer for ochratoxin A was immobilized onto magnetic nanospheres (MNS) and used to develop a magnetic solid-phase extraction procedure to clean up food samples in conjunction with high-performance liquid chromatography separation and fluorescence detection. Specific retention of ochratoxin A by the sorbent was demonstrated, and the capacity of the MNS-aptamer sorbent was determined. The efficacy of this new approach was successfully evaluated through comparison with solid-phase extraction on commercial C18 cartridge. Several different food samples fortified in the range of with 2.5-50μg/kg yielded mean recoveries from 67% to 90%, respectively. Finally, this oligosorbent was applied to the selective extraction of ochratoxin A from unfortified food samples.
Authors:
Ximei Wu; Jun Hu; Binghui Zhu; Lin Lu; Xiangdong Huang; Daiwen Pang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-19
Journal Detail:
Title:  Journal of chromatography. A     Volume:  -     ISSN:  1873-3778     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-9-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
College of Chemistry and Molecular Sciences, Wuhan University, Luojiashan, Wuhan 430072, China; Center for Disease Prevention and Control of Guangdong province, Guangzhou 510300, China.
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