| Aptamer-targeted magnetic nanospheres as a solid-phase extraction sorbent for determination of ochratoxin A in food samples. | |
| | |
MedLine Citation:
|
PMID: 21890142 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
A sorbent based on the aptamer for ochratoxin A was immobilized onto magnetic nanospheres (MNS) and used to develop a magnetic solid-phase extraction procedure to clean up food samples in conjunction with high-performance liquid chromatography separation and fluorescence detection. Specific retention of ochratoxin A by the sorbent was demonstrated, and the capacity of the MNS-aptamer sorbent was determined. The efficacy of this new approach was successfully evaluated through comparison with solid-phase extraction on commercial C18 cartridge. Several different food samples fortified in the range of with 2.5-50μg/kg yielded mean recoveries from 67% to 90%, respectively. Finally, this oligosorbent was applied to the selective extraction of ochratoxin A from unfortified food samples. |
| | |
Authors:
|
Ximei Wu; Jun Hu; Binghui Zhu; Lin Lu; Xiangdong Huang; Daiwen Pang |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-8-19 |
Journal Detail:
|
Title: Journal of chromatography. A Volume: - ISSN: 1873-3778 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
|
Created Date: 2011-9-5 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
|
College of Chemistry and Molecular Sciences, Wuhan University, Luojiashan, Wuhan 430072, China; Center for Disease Prevention and Control of Guangdong province, Guangzhou 510300, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A discontinuous Galerkin method to solve chromatographic models.
Next Document: Protein adsorption and transport in dextran-modified ion-exchange media. III. Effects of resin charg...